Prakash Kumar Bhagat

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Questions related from Prakash Kumar Bhagat

Can anyone explain the probable function of a protein if it is showing the auto activation in the Yeast two hybrid assay if it is not a TF ?

Hello everyone! this is quite common/rare observation that auto-activation of some genes apart from transcription factors are really a fascinating during Yeast two hybrid assay.But how can we...

04 April 2018 5,469 0 View

Hey everyone! If a protein is predicted to interact with the promoter of a gene, then which experiment will be more convenient or convince to explain?

Suppose a protein , i.e. Transcription factor, may be a regulator of a gene, in that case which one will be first experiment to further take ahead? Y1H, EMSA, ChIP, or in-vivo transactivation ?

12 December 2016 3,104 4 View