Kavita Rana

10 Questions 22 Answers 0 Followers

Questions related from Kavita Rana

I am new to western blot, i want to check nfkappa-b p65 protein in cell, i ran western blot, i have some doubts, regarding my results?

I am checking effect of my compound on breast cancer cells, i ran western blot, i am attaching photo, and i have following questions- 1- i have not used phosphatase inhibitor in sample preparation...

02 February 2020 6,652 3 View

I got diffused bands in western blott? What could be the reason?

I ran western blot, for bax (20kd), 15%gel, 100v , then transfer overnight 40V 4 degree, I am attaching photos ( ponceu/ chemiluminiscence)kindly help me out , why bands get diffused-

02 February 2020 3,156 2 View

Why sometimes mdamb231 cells grows in patterns like rows in-between in few places in flask??

I am new in cell culture techniques, I am maintaing 231mdamb cell lines right now, cells seems healthy and growing well, but I observe in one flask cells are growing in rows inbetween scatterted...

10 October 2019 1,373 5 View

How to distinguish cell debris and contamination?

I am culturing breast cancer cell line, some time I observe high density of tiny dot like structure, I am not able to find out whether its contamination or cell debris.if it is contamination then...

02 February 2019 7,044 1 View

I am facing problem in maintaining breast cancer cell lines?

I am beginner in mammalian cell culture, I am maintaining breast cancer cell line (t47d,mdamb231,mdamb468,mcf7). Cells were going well but suddenly i observed so much cell debris and slightly...

02 February 2019 9,867 4 View

Can we investigate Ic50 of drug without knowing its drug loading capacity in case of drug prepared with polymer?

I have prepared arsenic loaded nanoparticles now m looking forward 4 anticancer properties of this drug but i have nt calculated drug loading capacity yet..can i go straight for mtt assay to know...

12 December 2018 9,753 5 View

How to dissolve arsenic trioxide loaded plga nanoparticles ?

I have prepared arsenic trioxide loaded nanoparticles...now i have to calculate Ic50 for the same, can anybody suggest me which solvent would be best to dissolve these...

11 November 2018 5,930 1 View

I have to calculate encapsulation efficiency of Arsenic loaded PLGA nanoparticles?

From literature i got a formula = total amount of arsenic - free arsenic / total arsenic × 100%. Here free amount will be calculated after ultracentifugation of arsenic+plga...

08 August 2018 6,363 7 View

I am running sds page first time..without ot protein marker..i have taken 10 ulit (2mg/ml) of bsa protein + 2x loading dye?

After running gel for 2 and half hour at 90v , n staining with cumassie i got more then one band...what might be reason ? I am attaching pic.

08 August 2018 572 5 View

If i have protocols for tissue samples (kidney/liver) viz gsh,SOD,protein can i use them for cell culture(breast cancer cells)???

I have worked on invivo toxicity of liver kidney now i m shifting towards toxicity in mammalian cancer cell line. Can i use protocols i already have?

06 June 2018 9,584 5 View