What is the best way of processing very large files in python?

I want to process a very large file, let's say 300 GB, with Python and I'm wondering what is the best way to do it. One limitation is that my algorithm needs the whole file in memory, so a...

15 January 2021 6,691 4 View

Has anyone compared supervised classification methods using google earth engine?

Good Morning! Has anyone compared supervised classification methods using Google Earth Engine? Could you comment on what are the criteria you used to decide on any method?

29 November 2020 544 4 View

How can I find the correlation coefficient between 1 categorical variable compared to several other variables?

Let's say that I have 8 proteins: A-H and I have protein expression data for each gene. For each gene, the expression number is based on the average of 20 cell lines expressing that gene. I also...

10 May 2020 3,261 1 View

Can anyone clarify the difference between IC50 and dose to me?

I guess I'm not understanding the concept well. If I treated cells (5000/well) with a range of doses from 6nM to 92nM (and this is an MTT assay and I've used Prism8), why is the IC50 only .9nM? I...

24 February 2020 7,044 2 View

Is it really necessary the clean-up step after PCR for digesting with DpnI?

I've read different protocols, starting for the QuikChange one, and there's no call for cleaning up after PCR. However, I did clean up as suggested by other researchers, so the polymerase doesn't...

12 November 2019 9,677 5 View

Is it really necessary the clean-up step after PCR for digesting with DpnI?

I've read different protocols, starting for the QuikChange one, and there's no call for cleaning up after PCR. However, I did clean up as suggested by other researchers, so the polymerase doesn't...

10 November 2019 5,183 5 View

Protocol for polymer stainning with Ruthenium tetroxiode?

I cannot find a comprehensive protocol for stainning polymers with Ruthenium tetroxide solution. I would like to know the instructions and safety precautions for stainning and handling of...

04 November 2018 7,875 2 View

Are you interested in publishing in the Energies Special Issue "Optimization of Biodiesel and Biofuel Process"?

You can find proper information of this Special Issue in the following link: https://www.mdpi.com/journal/energies/special_issues/Biodiesel_and_Biofuel_Process

29 October 2018 1,429 21 View

How to determine the therapeutic indices for antimicrobial peptides?

I'm working with AMP's from scorpion venom and after determining the hemolytic activity, I woud like to be sure to determine properly the therapeutic index. Thanks

10 November 2017 740 0 View

Multitemporal landsat image?

29 October 2017 2,469 7 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

What are some important techniques for protein induction and SDS-PAGE analysis?

Hello everyone. I am fairly new in protein chemistry field. I am trying to perform a protein induction hoping to purify my protein of interest using FPLC method. However, I am having trouble on...

03 March 2021 8,237 3 View