How to perform a Tricine–SDS-PAGE?

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No correlation between the independent variables and the dependent variable?

I want to conduct a multivariable linear regression analysis with 15 independent variables, however when I was checking the assumptions not a single IV correlates with the dependent variable. Can...

14 May 2024 5,151 7 View

How much PCR product do I have to use for a Cas9 digestion?

I needed to do a Cas9 digestion of the amplicon, but I did not achieve it. Some protocols refer to the use of 1-2 uL of PCR product directly with optional purification, but I do not understand why...

16 September 2023 3,526 0 View

How to interpret the strength of association of the biserial correlation (rb)?

Hello, I estimated the biserial correlation between an artificial dichotomous variable and a continuous variable. But I want to know how to interpret the result in terms of strength of...

13 September 2023 2,528 0 View

How is the slurry applied on the mesh current collector ?

Electrode preparation / mesh current collector / battery / Active material

18 April 2023 2,679 2 View

Is it possible to work with a liquid cell in SEM, if yes how? Is there a special liquid-cell holder for it ?

SEM/ Liquid-cell

18 April 2023 6,611 3 View

What is the difference between a liquid cell and a battery ?

Liquid cell Vs Battery

13 April 2023 3,555 3 View

How does one determine the sex of soft ticks (Argasidae)?

Sex in hard ticks (Ixodidae) is very easy to determine, but does anyone have a reliable method to determine the sex in soft ticks (Argasidae)? Thanks in advance. Manuel

16 February 2023 5,866 2 View

Is the in-situ raman pouch cell used only once ? and cannot be disassembled and assembled again for a second use ?

Unlike the ampix cell used for in-situ operando, can we disassemble the pouch cell and the coin cell and assemble them for a second use ?

20 January 2023 5,048 0 View

Why do we need an anode with high capacity and low voltage and a cathode with high capacity and high voltage to have a good chemistry in a battery?

Good evening everyone, I just started working on my end of year thesis. The project is about in-situ characterization of batteries using xrd, raman and sem. Since I am new in the battery field I...

07 January 2023 6,882 4 View

How to quantitatively measure knowledge management?

*UPDATE* Hello everyone! I'm looking for papers that demonstrate/show how to quantitatively measure knowledge management (in general or part of it, like KM processes). In particular, I need to...

30 December 2022 2,406 16 View

How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...

08 August 2024 8,329 0 View

Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

How to preform densitometry on SDS-page bands?

I ran a SDS-page of a bacterial lysate and I want to quantify protein concentration in a specific band. I was thinking of using a standards ladder or make some standards are different...

05 August 2024 9,805 3 View

How can I select cells in prime editing without fluorescence-activated cell separation?

31 July 2024 8,551 2 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Following click reaction in cell lysates, protein is immobile and remains at the top of the gel in SDS-PAGE?

I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...

29 July 2024 950 4 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View