Catherine Cesa-Luna

3 Questions 8 Answers 0 Followers

Questions related from Catherine Cesa-Luna

Is it really necessary the clean-up step after PCR for digesting with DpnI?

I've read different protocols, starting for the QuikChange one, and there's no call for cleaning up after PCR. However, I did clean up as suggested by other researchers, so the polymerase doesn't...

11 November 2019 5,484 5 View

How to determine the therapeutic indices for antimicrobial peptides?

I'm working with AMP's from scorpion venom and after determining the hemolytic activity, I woud like to be sure to determine properly the therapeutic index. Thanks

11 November 2017 917 0 View

How to perform a Tricine–SDS-PAGE?

How to prepare the gel? What are the best running conditions for small proteins?

03 March 2017 6,207 2 View