I needed to do a Cas9 digestion of the amplicon, but I did not achieve it. Some protocols refer to the use of 1-2 uL of PCR product directly with optional purification, but I do not understand why the protocols indicate that the molar ratio has to be 10:10:1 (sgRNA:Cas9:PCR product) and at the same time also refer to the use of only 1-2 uL of PCR product for the digestion.

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