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To determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a 10 mg/mL lipid concentration, consider the lipid-to-siRNA weight ratio. Common ratios range from 5:1 to 20:1 [1][2]. With a 10:1 ratio, you have 25 mg of lipids (2.5 mL * 10 mg/mL), allowing for 2.5 mg of siRNA. Given the siRNA concentration, calculate the corresponding volume. For example, if the siRNA is at 1 mg/mL, you can add up to 2.5 mL of siRNA solution. This assumes optimal encapsulation and stability conditions [3][4].

Reference

[1] Evers, M., Wakker, S. I. v. d., Groot, E. M. d., Jong, O. G. d., Gitz-Francois, J. J., Seinen, C. S., Sluijter, J., Schiffelers, R., & Vader, P. (2021). Functional siRNA Delivery by Extracellular Vesicle–Liposome Hybrid Nanoparticles. Advanced Healthcare Materials, 11.

[2] Yu, Q., Zhang, B., Zhou, Y., Ge, Q., Chang, J., Chen, Y., Zhang, K., Peng, D., & Chen, W. (2019). Co-delivery of gambogenic acid and VEGF-siRNA with anionic liposome and polyethylenimine complexes to HepG2 cells. Journal of Liposome Research, 29, 322 - 331.

[3] Yu-Wai-Man, C., Tagalakis, A., Manunta, M., Hart, S., & Khaw, P. (2015). Receptor-targeted liposome-peptide-siRNA nanoparticles represent a novel and efficient siRNA delivery system to prevent conjunctival fibrosis. Acta Ophthalmologica, 93.

[4] Li, L., & An, X. (2016). A novel combined method of thin-film evaporation and a supercritical carbon dioxide technique to prepare a fluorescent siRNA-liposome. RSC Advances, 6, 92115-92119.