How can I select cells in prime editing without fluorescence-activated cell separation?

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Hello all Can we use different concentrations of lemon or orange extract to grow flowers in ornamental trees and shrubs?

I need more pdf on this topic

02 July 2024 6,988 1 View

Is there a close relationship between the glassbrush bush and its insect pollinator, the honey bee?

I need more information about this topic

27 June 2024 4,057 3 View

How do bees work on glassbrush flowers?

I need pdf information to increase my knowledge of this pollinator, which is honeybees

27 June 2024 3,815 1 View

Welcome What is the scientific name of this climbing plant?

I was confused about the scientific name as well as the local name because of the great similarity between this plant and another climbing plant, especially the morphological shape, which is very...

22 June 2024 9,570 2 View

Hello all Do peanut shell residues preserve soil moisture after planting?

I want to confirm the information so I can add it to my simple information

20 June 2024 4,160 0 View

Welcome Find these basic axes in p df form:1.Introduction to Botany:2.Plant Structure and Function?

conservation Conclusion: In summary, "Exploring Botany: An Inclusive Guide to Plant Biology" aims to be an invaluable resource for students, educators, and enthusiasts interested in delving into...

13 June 2024 5,807 1 View

Hello all I am currently working in extracting plant parts using ultrasound. Can I use the GC-MS technology or chromatographic analysis?

A number of active compounds and their concentrations found in flower and leaf extracts were obtained using this technique

09 June 2024 1,872 4 View

Why does the aloe vera gel change color from white to pink when placed in the refrigerator?

While when placed in the freezer, it maintains the same original white color?

05 June 2024 5,231 5 View

Welcome Do I need a free Scopus indexed magazine?

I have an English research paper that I need to publish

05 June 2024 6,772 0 View

S there any classified research on the glass brush bush?

I need this research in PDF format

02 June 2024 661 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View