I am evaluating the potential anti-invasive effect of cytotoxic compound on breast cancer cells, MDA-MB-231 and SK-BR-3. I ordered Cultrex 96 well BME invasion kit and the manufacturer recommend that the cells should be passaged 2 or 3 times prior to use. My question is do they mean we should use cell that passeged only 2-3 times after thawing the original vial or we can use cells that had been passaged several times (for example, more than 10 times) in order to confirm their viability and growth pattern?
Thanks in advanced
Hi, Fawaz. I have used both of those cell lines and they are pretty stable cell lines. I'm not completely sure, but I would think the 2-3 passages would be just to get the growth stable after thawing from nitrogen. I have always used my cells up until passage number 30. You could always call tech support for the kit just to be sure.
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