Hello everybody,

I am currently extracting total RNA including microRNA from serum using miRNeasy Serum/ Plasma kit Catalog Number 217184 from Qiagen. Within the procedure, we add a Spike -In control to test the recovery of RNA extracted using RT-PCR. The next steps will be library preparation and sequencing using Illumina Machines. Do you expect that the Spike in control will have an effect on the results?

many thanks in advance,

Fawaz