How much of TNF-Alpha is added to transform THP-1 into M1 macrophage?

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Vinod Nadella

THP-1 cells can be differentiated into macrophages using PMA. Further, these cells can be incubation with LPS and IFN-γ or TNF-α for classical macrophage activation (M1).

TNF-α at 10 ng/ml will do the job

Fawaz Al-Shaheri

Thank you very much.

Murad Alahdal

according to my experience, 10-20ng/mL of TNF-α can be used for M1 differentiation of THP-1

@Murad Alahdal,

Thanks for your nice comment.

Rahmat Dani Satria

1.How many THP1 need

2. how many hours THP1 need incubate with TNF alpha