Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View

What could be the possible causes of DNA degradation after extraction from 4% gel?

Hi everyone, During small RNA library preparation, I run the samples in 4% agerose gel to select the band corresponding to my target. I established beforehand the protocol and it works fine....

02 February 2021 5,008 2 View

What is this citation style called?

Hello everyone, I am preparing a manuscript for a journal which indicates in the authors guidelines that the citation and referencing style should look like the following: Citation in the text...

22 September 2020 6,897 6 View

What would be the effect of Spike-In control in microRNA Next Generation Sequencing results?

Hello everybody, I am currently extracting total RNA including microRNA from serum using miRNeasy Serum/ Plasma kit Catalog Number 217184 from Qiagen. Within the procedure, we add a Spike -In...

07 August 2019 5,494 3 View

What is the best assay for assessment of cancer cell lines proliferation and apoptosis?

I working with pancreatic cancer cell lines PT45P1 and would like to know whether there is a better test rather than wound healing assay to assess the cellular proliferation after and before...

07 August 2019 8,401 10 View

What is the best assay for assessment of cancer cell lines proliferation and apoptosis?

I working with pancreatic cancer cell lines PT45P1 and would like to know whether there is a better test rather than wound healing assay to assess the cellular proliferation after and before...

06 August 2019 6,195 9 View

How we can prepare labeling, washing and incubation buffers for antibody micro-array?

I need to prepare a washing, incubation and labeling buffers for antibody microarray. Anyone has idea how to prepare them?

09 October 2018 6,505 1 View

What the best protocol for protein extraction from human serum?

I am planning to extract the whole serum protein from serum samples for further microarray analysis. what is your recommendations please. a good protocol is highly appreciated. Thanks in advance

16 September 2018 3,896 3 View

What the best protocol for protein extraction from human serum?

I am planning to extract the whole serum protein from serum samples for further microarray analysis. what is your recommendations please. a good protocol is highly appreciated. Thanks in advance

08 September 2018 9,076 5 View

How can we check the specificity of methylation Specific Primers?

I am studying the methylation status of APC gene in colorectal patients. My practical approach starts with bisulfite conversion of DNA and then amplification using methylation specific PCR....

10 September 2017 2,639 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View