Fawaz Al-Shaheri

12 Questions 36 Answers 0 Followers

Questions related from Fawaz Al-Shaheri

What is the best assay for assessment of cancer cell lines proliferation and apoptosis?

I working with pancreatic cancer cell lines PT45P1 and would like to know whether there is a better test rather than wound healing assay to assess the cellular proliferation after and before...

08 August 2019 8,492 10 View

What would be the effect of Spike-In control in microRNA Next Generation Sequencing results?

Hello everybody, I am currently extracting total RNA including microRNA from serum using miRNeasy Serum/ Plasma kit Catalog Number 217184 from Qiagen. Within the procedure, we add a Spike -In...

08 August 2019 5,578 3 View

What is the concentration of Gemcitabin that I should used to treat PT45P1 pancreatic cancer cell lines?

Hello everyone, Is there anyone has experience in treating PT45P1 pancreatic cancer cell lines with Gemcitabin? I would really appreciate it very much if you let me know how much the Concentration...

08 August 2019 5,823 3 View

How we can prepare labeling, washing and incubation buffers for antibody micro-array?

I need to prepare a washing, incubation and labeling buffers for antibody microarray. Anyone has idea how to prepare them?

10 October 2018 6,594 1 View

What the best protocol for protein extraction from human serum?

I am planning to extract the whole serum protein from serum samples for further microarray analysis. what is your recommendations please. a good protocol is highly appreciated. Thanks in advance

09 September 2018 9,168 5 View

How can we check the specificity of methylation Specific Primers?

I am studying the methylation status of APC gene in colorectal patients. My practical approach starts with bisulfite conversion of DNA and then amplification using methylation specific PCR....

09 September 2017 1,616 2 View

How many times the cell should be passaged in order to use in an invasion assay?

I am evaluating the potential anti-invasive effect of cytotoxic compound on breast cancer cells, MDA-MB-231 and SK-BR-3. I ordered Cultrex 96 well BME invasion kit and the manufacturer recommend...

04 April 2017 6,482 2 View

What is the cause of nonspecific bands in methylation specific PCR?

I am working in the methylation status of some genes in cancer patients. We recruited clinical samples from patients with and without cancer. We designed methylation specific primer and...

01 January 1970 10,007 3 View

Where we can find Colorectal Adenoma cell lines?

We need pre-malignant colorectal adenoma cell lines to study the effect of mutation in malignant transformation(tubular adenoma, villous adenoma and tubuvillous adenoma cell lines). I have been...

01 January 1970 1,993 4 View

What is the most suitable restriction enzyme we should use to insert the MUTYH into plasmid vector pET+25b?

I am planning to do functional analyses for a novel mutation to investigate its effect using cell based cloning. The data from literature indicate that pET+25b is the best vector to create the...

01 January 1970 3,508 4 View

What is culture filtrate ?

I have been reading this term many times in my handouts .But ,I don't know what does it mean .

01 January 1970 3,543 2 View

How we can improve the DNA concentration extracted from aged samples?

We are extracting DNA from aged blood samples using Qiagen DNA extraction Kit. Unfortunately, the DNA concentration that we've got is 5-6 microgram/ microliters at 260/280. Do you have any...

01 January 1970 3,479 6 View