regards
Jitendra
I used CCk-8 kit (works on dehydogenase activity in viable cells) to do cytoxicity assay and I got higher absorbance (@450 nm, reference- 650 nm) for my tests than control (cells in microwell...
03 April 2016 8,760 2 View
I want to perform MTT assay to prove application of a certain drug for biomedical application in general (not oral dose), can I use any mammalian cell lines? Thanks
11 December 2015 6,426 2 View
Thanks and regards Jitendra
11 December 2014 5,227 5 View
Why bacterial system is still used for protein production for some protein knowing that it doesn't have a system to support post translational modification?
11 December 2014 6,122 5 View
I used 20 microL of transformed DH5-a cells (pUC18 transformed, grown overnight in liquid LB after vial thawing) and spread it on LB agar (amp plate-100microL/ml) plate but was surprised to see...
10 November 2014 4,787 4 View
I was wondering if there is a fixed pI for all monoclonal antibodies as most of the labs use glycine and phosphate buffer systems for MAb isolation using Protein A-Affinity (in HPLC).
10 November 2014 4,823 3 View
Please elaborate the change in Enzyme kinetics (v, Km etc) between an immobilized and an enzyme in solution. Thanks and regards Jitendra
10 November 2014 3,552 15 View
If yes, then what effect does it impart?
09 October 2014 8,313 3 View
Thanks
09 October 2014 2,280 10 View
Has anyone ever tried something new during the plasmid isolation from bacteria cells which resulted in improved plasmid yield? Suggestions can cover centrifugation, changing the concentration of...
08 September 2014 9,437 7 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...
27 July 2024 6,605 0 View
I use 0.5% sarkosyl to solubilise recombinant proteins contained in Inclusion bodies of E. coli. sarkosyl very effeciently solubilizes proteins from Inclusion bodies and I am trying to use...
25 July 2024 5,001 2 View
"The Correlation between Nutrition and Transport Mechanism under Abiotic Stress in Plants: A Comprehensive Review" by Muhammad Saleem, Jianhua Zhang, Muhammad Qasim, Rashid Iqbal, and Li Song,...
23 July 2024 9,109 0 View
Good morning, I am trying to produce a recombinant protein. Unfortunately I one of the two restriction enzymes that I use do not cut at the end of the MCS. After the digestion 15 nucleotides of...
17 July 2024 2,787 4 View
I have used Bradford assay for protein quantification; however, I am getting inconsistent concentrations. Tried twice but similar inconsistency. Like the highest quantity of a biomaterial is...
11 July 2024 2,257 1 View
I have used Bradford assay for protein quantification; however, I am getting inconsistent concentrations for the second time. Like the highest quantity of a biomaterial is having lower...
11 July 2024 3,557 3 View
I have hemostatic nanofibers of hydrophilic nature. While fibrin formation from fibrinogen can be qualitatively visualized using SEM. What assays can be performed on nanofibers to quantify the...
08 July 2024 3,714 1 View
My protein is a transmembrane protein, therefore i need to construct a transmembrane using CHARMM-GUI. Can someone please suggest me a tutorial for the same.
24 June 2024 5,044 1 View
