Is it normal to get higher cell activity in test during Cytotoxicity assay

I used CCk-8 kit (works on dehydogenase activity in viable cells) to do cytoxicity assay and I got higher absorbance (@450 nm, reference- 650 nm) for my tests than control (cells in microwell...

03 April 2016 8,631 2 View

For MTT assay, how do you select cell line?

11 December 2015 6,289 2 View

Difference between silk produced by spider and silkworm?

regards Jitendra

11 December 2014 9,589 0 View

What are the merits/demerits in using S-nitroso human serum albumin for cancer treatment?

Thanks and regards Jitendra

11 December 2014 5,079 5 View

Why bacterial system is still used for protein production?

Why bacterial system is still used for protein production for some protein knowing that it doesn't have a system to support post translational modification?

11 December 2014 5,971 5 View

What could be the reason of getting a lawn of transformed cells on LB plate?

10 November 2014 4,636 4 View

What happens to the enzyme kinetics when in solution and when immobilized?

10 November 2014 3,400 15 View

Do epithelial cancer cells release nitric oxide?

If yes, then what effect does it impart?

09 October 2014 8,166 3 View

Why is it important to know the protein conc. accurately to know the protein structure using circular dichroism?

Thanks

09 October 2014 2,142 10 View

Has anyone ever tried something new during the plasmid isolation from bacteria cells which resulted in improved plasmid yield?

Has anyone ever tried something new during the plasmid isolation from bacteria cells which resulted in improved plasmid yield? Suggestions can cover centrifugation, changing the concentration of...

08 September 2014 9,297 7 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

I have designed reflectarray element and feeding it though Horn Antenna on HFSS. After full wave simulation, I am not getting desired results?

I have designed reflectarray element to generate OAM beams at 5.8GHz and feeding it though Horn Antenna on HFSS. After full wave simulation, i am not getting desired results i.e Radiation pattern...

01 March 2021 9,701 5 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View