How can we quantify protein in a natural biomaterial which is a blend of proteins, carbohydrates, etc?

12 July 2024 1 2K Report

I have used Bradford assay for protein quantification; however, I am getting inconsistent concentrations. Tried twice but similar inconsistency. Like the highest quantity of a biomaterial is having lower concentration of protein than that of the lower quantity. For example, 2mg of biomaterial have 302.3 ug/ml conc. while 2.5mg has 344.2ug/ml conc. of protein and so on.

Omar Gonzalez-Ortega

First of all, Bradford's method sucks. Second, the response is not linear. Third, what is the standard deviation of your analysis? Fourth, if the biomaterial has something that interferes with the assay (surfactants of something similar) you need to switch to electrophoresis for example.

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