Jitendra Pant

19 Questions 54 Answers 0 Followers

Questions related from Jitendra Pant

Is it normal to get higher cell activity in test during Cytotoxicity assay

I used CCk-8 kit (works on dehydogenase activity in viable cells) to do cytoxicity assay and I got higher absorbance (@450 nm, reference- 650 nm) for my tests than control (cells in microwell...

04 April 2016 8,727 2 View

For MTT assay, how do you select cell line?

I want to perform MTT assay to prove application of a certain drug for biomedical application in general (not oral dose), can I use any mammalian cell lines? Thanks

12 December 2015 6,383 2 View

Difference between silk produced by spider and silkworm?

regards Jitendra

12 December 2014 9,673 0 View

Why bacterial system is still used for protein production?

Why bacterial system is still used for protein production for some protein knowing that it doesn't have a system to support post translational modification?

12 December 2014 6,076 5 View

What are the merits/demerits in using S-nitroso human serum albumin for cancer treatment?

Thanks and regards Jitendra

12 December 2014 5,176 5 View

What could be the reason of getting a lawn of transformed cells on LB plate?

I used 20 microL of transformed DH5-a cells (pUC18 transformed, grown overnight in liquid LB after vial thawing) and spread it on LB agar (amp plate-100microL/ml) plate but was surprised to see...

11 November 2014 4,732 4 View

What is the pI of a monoclonal antibody?

I was wondering if there is a fixed pI for all monoclonal antibodies as most of the labs use glycine and phosphate buffer systems for MAb isolation using Protein A-Affinity (in HPLC).

11 November 2014 4,788 3 View

What happens to the enzyme kinetics when in solution and when immobilized?

Please elaborate the change in Enzyme kinetics (v, Km etc) between an immobilized and an enzyme in solution. Thanks and regards Jitendra

11 November 2014 3,507 15 View

Why is it important to know the protein conc. accurately to know the protein structure using circular dichroism?

Thanks

10 October 2014 2,237 10 View

Do epithelial cancer cells release nitric oxide?

If yes, then what effect does it impart?

10 October 2014 8,266 3 View

Should we use over night culture for Plasmid DNA isolation from DH5-alpha?

Hello friends, I wanted to know in which case there is a better probability (practically not theoritically) to get more plasmid yield? A) Pickup a transformed colony and put it for 12-16 hrs...

09 September 2014 4,204 8 View

How can I make sure that ss staple hybridizes with ss DNA to get the desired origami?

I want to know how can we make sure that after heating staple and ds DNA, how can I make sure that the ss DNA hybridizes with ss staple and not to their own complementary strand again (i.e ss DNA...

09 September 2014 8,249 2 View

How can we make the proteins using Cell-free display, starting with mRNA directly?

Cell-free Display protein. Does it affect the protein folding?

09 September 2014 2,255 4 View

Why is Circular Dichroism a better tool to study effect of point mutation on protein stability as compared to fluorescence spectrometry?

I would also like to know how you would differentiate between the point mutation results in the same amino acids? Thanks for the help. regards Jitendra

09 September 2014 9,907 6 View

Has anyone ever tried something new during the plasmid isolation from bacteria cells which resulted in improved plasmid yield?

Has anyone ever tried something new during the plasmid isolation from bacteria cells which resulted in improved plasmid yield? Suggestions can cover centrifugation, changing the concentration of...

09 September 2014 9,389 7 View

Does anyone have experience of working with reporter-promoter construct?

Reporter-promoter construct and transfection. I want to design such a construct for checking the activity of AP1. I would also like to know if calcium phosphate method of transfection is good...

08 August 2014 3,843 4 View

What techniques are better than 16s rRNA ribotyping to draw phlogenetic relationship?

Please tell me the biggest disadvantages of using 16s rRNA for phylogeny and techniques, which are better in this regard. I would appreciate if someone can also send me papers to read on...

08 August 2014 6,250 4 View

Why is lysine eluted in the end in reverse phase chromatography (HPLC, c-18 column)?

Reverse phase chromatography utilizes the hydrophobic interactions between the column and the amino acids. Therefore, theoretically polar amino acids should be eluted first while more hydrophobic...

08 August 2014 7,628 3 View

What is the difference between alpha/beta and alpha +beta protein?

Please quote examples as well. Thanks in advance.

08 August 2014 6,297 6 View