25 June 2024 1 5K Report

My protein is a transmembrane protein, therefore i need to construct a transmembrane using CHARMM-GUI. Can someone please suggest me a tutorial for the same.

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Z-average size from Dynamic Light scattering can it be lower than the original probe size?

Hi everyone, Recently I have been conducting Dynamic Light scattering experiments in a micellar solution at 5 and gel at 37 degrees of Celsius with latex particles of diameter 190-500 nm. While...

01 August 2024 1,168 4 View

Method for estimation of biomass using non-destructive method for ornamental plants?

Allometric equations are available for trees but couldn't find any method/equation for ornamental plants?

04 July 2024 2,646 1 View

Is there any equipment that replaces GPC/SEC in carrageenan characterization?

Is there any equipment that replaces GPC/SEC in carrageenan characterization?

22 June 2024 2,361 3 View

Does anyone have this article in PDF and could they pass it on to me?

Paper: Wang, A.G., Luo, G.H., 1990. Quantitative relation between the reaction of hydroxylamine and superoxide anion radicals in plants. Plant Physiol. Commun. 6, 55–57.

19 June 2024 7,283 0 View

Viscosity or Complex viscosity should be used in the SOP for Malvern Zetasizer or carrying out DLS experiments for polymer samples?

Hi all, I am trying to conduct Dynamic Light scattering experiments on polymer sample using Malvern Zetasizer ZS. One question I have is to whether it is okay to use complex viscosity instead of...

11 May 2024 4,623 7 View

Crispr knockout validation?

I am doing crispr knockout in T cells. After transfection, I am trying to validate knockout by western blot and sequencing. with western blot, I can see truncated protein in knockout cells whereas...

30 March 2024 4,408 2 View

Crispr knockcout validation?

I am doing crispr knockout in T cells. After transfection, I am trying to validate knockout by western blot and sequencing. with western blot, I can see truncated protein in knockout cells whereas...

29 March 2024 5,969 1 View

Is energy minimisation necessary step before molecular docking for protein-ligand interaction?

I have to perform molecular docking on 2 ligands (A structure-absent in literature and B structure-sourced from PubChem) therefore, is energy minimisation necessary for both?

14 March 2024 7,560 3 View

Can anyone provide suggestions for anesthesia in post-natal/ juvenile mice (P10-P16)?

I want to do stereotactic surgery/ microinjection in post-natal/juvenile mice within the age range of P10-P16. I am specifically looking for suggestions on anesthesia. The pup does not get...

10 March 2024 7,785 2 View

How can I put AutoDock4 reading my file?

Good afternoon, I am using AutoDock4 to do docking of a protein with 2 cobalts in the active site. I added the parameters of the cobalt in both the AD4_parameter.dat file and to the...

14 January 2024 6,033 0 View

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Transfection in HEK293T cells?

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How to retain the a GFP tagged gene expression in stable cell line?

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What is the relationship between protein structure and N or C terminal tagging choosing?

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