For MTT assay, how do you select cell line?

11 December 2015 6,289 2 View

Difference between silk produced by spider and silkworm?

regards Jitendra

11 December 2014 9,589 0 View

What are the merits/demerits in using S-nitroso human serum albumin for cancer treatment?

Thanks and regards Jitendra

11 December 2014 5,079 5 View

Why bacterial system is still used for protein production?

Why bacterial system is still used for protein production for some protein knowing that it doesn't have a system to support post translational modification?

11 December 2014 5,971 5 View

What could be the reason of getting a lawn of transformed cells on LB plate?

10 November 2014 4,636 4 View

What is the pI of a monoclonal antibody?

I was wondering if there is a fixed pI for all monoclonal antibodies as most of the labs use glycine and phosphate buffer systems for MAb isolation using Protein A-Affinity (in HPLC).

10 November 2014 4,700 3 View

What happens to the enzyme kinetics when in solution and when immobilized?

10 November 2014 3,400 15 View

Do epithelial cancer cells release nitric oxide?

If yes, then what effect does it impart?

09 October 2014 8,166 3 View

Why is it important to know the protein conc. accurately to know the protein structure using circular dichroism?

Thanks

09 October 2014 2,142 10 View

Has anyone ever tried something new during the plasmid isolation from bacteria cells which resulted in improved plasmid yield?

Has anyone ever tried something new during the plasmid isolation from bacteria cells which resulted in improved plasmid yield? Suggestions can cover centrifugation, changing the concentration of...

08 September 2014 9,297 7 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

No title

02 March 2021 3,060 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View