I have used Bradford assay for protein quantification; however, I am getting inconsistent concentrations for the second time. Like the highest quantity of a biomaterial is having lower concentration of protein than that of the lower quantity. For example, 2mg of biomaterial have 302.3 ug/ml conc. while 2.5mg has 344.2ug/ml conc. of protein and so on.
Quantifying the entirety of proteins from a complex mixture is nearly impossible, as they come in all forms and sizes and compositions. Typically, Bradford does a good job, but even good ol' Coomassie has it's bias. BCA is said to have less bias towards impurities.
Dear all, the following recent review compares the different methods involved in determining proteins content in carbohydrates. My Regards
10.3390/foods9101340
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