What is the reason film thickness increased?

what is the reason film thickness increased when increased doping concentrations give some reasons The measured film thickness is 156, 282, 394, 548 and 681 nm for 0, 1.5, 2.5, 3.5, and 4.5 wt....

17 February 2021 8,987 5 View

BIM Conferences/ Symposium 2021?

Hi, I am Looking for BIM conferences and Symposiums for the year 2021, in order to send my paper. Please do let me know if anyone knows where I can find a list of upcoming Events in BIM. Thank you

30 November 2020 3,978 9 View

Which tool can be used to identify direct (or) inverted repeats in a gene?

I am trying to find out the presence of short repeats (both direct and inverted) in a 3-4 kb long DNA fragment. Although I had tried with a few free online tools, the results were not...

16 October 2020 8,368 3 View

Can the same data be used for both within group correlations and between group mean comparisons?

I have two different measures from two different groups. Both M1 (measure 1) and M2 (measure 2) from both G1 (group 1) and G2 (group 2). Within each group, I want to find correlation between M1...

08 October 2020 1,847 5 View

What are the possible by products from industries manufacturing mono clonal antibodies?

What are the possible by products from industries manufacturing mono clonal antibodies?

31 August 2020 7,124 2 View

Colour and solubility of poly(ethylene adipate) and poly(butylene adipate)?

I would like to know the colour of these two aliphatic polyesters. Also, I am curious to know their solubility in common organic solvents? Are these materials soft solid for feel? Can be they made...

16 August 2020 4,169 2 View

Can someone please tell me the color of the compound DDQH2 and its solubility nature ?

I have gone through all the online sources and vendor sites to find out the physical appearance of DDQH2 but I did not find any answers. Any kind of information on physical appearance, solubility,...

16 August 2020 8,822 2 View

A black colored layer is forming n the surface of NiCrAlY target during sputtering. Does it affect deposition?

I'm using a magnetron sputtering system to deposit thin-film sensors like strain gauge. Now I'm in the stage of bond coat trials. During the process, a black colored layer is forming on the...

09 February 2020 3,899 1 View

Are there any research on practices of clinical psychologists/mental health professionals in India?

Any data on the mentioned topic are welcomed.

30 January 2020 3,470 2 View

Is it possible to do a lentiviral infection/transduction on trypsinized cells still in cell suspension while plating them in Exp plate?

I have a lentivirus (VSV-G) containing an enhancer and general promoter coupled to a fluorescent protein-encoding gene (mCherry). The vector is used as a tool to identify factors able to stimulate...

20 January 2020 3,115 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Can random sampling have inclusion criteria?

As random sampling means that everyone in the population have an equal chance to be chosen, can it have inclusion criteria? My research participants have been chosen based on the inclusion...

26 February 2021 6,905 10 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

Is it possible to quantify recombinant protein in the supernatant using SDS PAGE ?

I would like to quantify a recombinant protein (with native signal sequence) secreted by Pichia pastoris using the SDS PAGE. Since my standard protein (plant origin) is not pure, I would like to...

07 February 2021 3,880 9 View

How to measure the concentration of a labeled protein with no tryptophan or tyrosines?

Hey I'm trying to measure the concentration of a protein I've purified and then labeled with Atto 647 flourescent dye. The protein has no tryptophans or tyrosines (although does have...

04 February 2021 4,482 4 View

Do you consider it is important to vindicate Spanish as language, in scientific issues and publication of scientific articles?

03 February 2021 3,756 4 View

How to prepare protein samples in EMSA assay?

Recently l was studying EMSA assay, but l didn't find any detailed information about protein sample. we can use two kinds of protein, including crude protein extracts and recombinant proteins. but...

02 February 2021 10,047 3 View

Difference between assessing EC50 in presence of physiological levels of human serum albumin and alpha1 acid glycoprotein vs. % human serum?

01 February 2021 1,825 1 View