Is it normal to get higher cell activity in test during Cytotoxicity assay

I used CCk-8 kit (works on dehydogenase activity in viable cells) to do cytoxicity assay and I got higher absorbance (@450 nm, reference- 650 nm) for my tests than control (cells in microwell...

03 April 2016 8,631 2 View

For MTT assay, how do you select cell line?

11 December 2015 6,289 2 View

Difference between silk produced by spider and silkworm?

regards Jitendra

11 December 2014 9,589 0 View

What are the merits/demerits in using S-nitroso human serum albumin for cancer treatment?

Thanks and regards Jitendra

11 December 2014 5,079 5 View

Why bacterial system is still used for protein production?

Why bacterial system is still used for protein production for some protein knowing that it doesn't have a system to support post translational modification?

11 December 2014 5,971 5 View

What is the pI of a monoclonal antibody?

I was wondering if there is a fixed pI for all monoclonal antibodies as most of the labs use glycine and phosphate buffer systems for MAb isolation using Protein A-Affinity (in HPLC).

10 November 2014 4,700 3 View

What happens to the enzyme kinetics when in solution and when immobilized?

10 November 2014 3,400 15 View

Do epithelial cancer cells release nitric oxide?

If yes, then what effect does it impart?

09 October 2014 8,166 3 View

Why is it important to know the protein conc. accurately to know the protein structure using circular dichroism?

Thanks

09 October 2014 2,142 10 View

Has anyone ever tried something new during the plasmid isolation from bacteria cells which resulted in improved plasmid yield?

Has anyone ever tried something new during the plasmid isolation from bacteria cells which resulted in improved plasmid yield? Suggestions can cover centrifugation, changing the concentration of...

08 September 2014 9,297 7 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View