I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone provide tips on how to avoid preferential paths?
In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...
01 August 2024 4,403 4 View
Hello, good morning. I would like to know which plate I can use to infect fresh PBMC with virus and culture for 24 hours. I have tried using 12-well and 24-well plates, but many cells adhere and...
24 July 2024 2,158 2 View
I am working with microplastics and need to analyze their color, size, shape, and quantity in sand samples. We require a technique that can reliably identify these characteristics. Could you...
13 June 2024 7,216 3 View
The mid-stance or mid-support has been defined as much as a phase or event within the race cycle. However, it did not identify a specific definition or a biomechanical indicator that would allow...
10 June 2024 6,257 2 View
I am tring to culture cells in hypoxia using Cobalt chloride model , but I want to compare with antoher method. Our research is about circRNA expression - qPCR and nanopore oxford sequencing.
05 June 2024 2,246 1 View
Hi, I'd like to know if someone knows about a company where I can buy an affordable laser-scriber for perovskite films and another thin film for the fabrication of modules. Many thanks,
31 May 2024 2,841 0 View
As keys in input, I used: %Mem=20GB %Nproc=16 %chk=etilantraquinona_professor_func=6-31G_Au_Norte.chk #B3LYP/GEN OPT FREQ=RAMAN Nosymm PSEUDO=READ SCF=TIGHT" **** 0 2 . . . . C O H...
17 May 2024 391 2 View
Regarding the nomenclature of galactans found in agarophytes, which is the correct name for the polysaccharide: "agaran" or "agar"? Why is there so much confusion about the use of this term?
27 April 2024 4,621 3 View
It's never used... Then the bodies fall in, right?
27 March 2024 6,696 1 View
Is there a direct and simple relationship between the solubility of a salt and the dielectric constant of the solvent or solvent mixture in which the salt is to be solubilised? For example: the...
21 March 2024 2,313 2 View
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Why is TLC not a form of partition chromatography and paper chromatography not a form of absorption chromatography?
03 August 2024 7,035 3 View
or we can collaborate
01 August 2024 6,705 2 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
I need something like Camag's VisionCat software for photodocumentation of TLCs. Could you recommend something?
31 July 2024 8,415 1 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View