Hi I am trying to developing indirect ELISA using antibody and recombinant protein (still finding).
For protein stadndard I found some recombinant protein product from variety manufacture, but I saw that some of their product is not full length product. Some product has only half of their original size (25kD original size but the product is only 11kD).
In this case, is it same amount when same mass of these protein fragment or partial protein (I am sorry, I don't know what to say) and full length protein was used.
I meand when I load 2ug of these protein fragment and full length protein, could I get same signal in ELISA? or Could I use these partial protein as my protein standard for ELISA?