I placed mice in metabolic cages to collect urine samples for analysis. However, the majority of the samples have dried up. Is there any way to retrieve the urine in those tubes without interfering with the biochemical properties of the urine?
My study uses C57BL/6J mice and I am required to store portions of the important organs in neutral buffered formalin (NBF). Could I potentially keep those tissues in the formalin for a few weeks...
06 July 2024 8,360 2 View
I am currently working towards developing a model for type 2 diabetes, using fructose and streptozotocin (STZ). Prior to injecting my C57BL/6 mice with STZ, we tested blood glucose with a...
08 May 2024 3,947 2 View
I am working with C57BL/6 mice for the development of a T2DM mouse model. During the course of this experiment, the mice will have their urine, blood, and tissue samples collected and examined for...
16 April 2024 530 2 View
The four diabetic microvascular complications I am assessing are nephropathy, neuropathy, cardiomyopathy, and retinopathy. Are there any in silico methods which can be employed to identify...
16 April 2024 1,433 0 View
My study focuses on developing a mouse model for the type II diabetes, narrowing in on a condition resembling chronic, late-stage diabetes. As such, blood glucose levels would need to be measured...
23 October 2023 8,110 4 View
My project aims at producing a reproducible mouse model for T2DM and its related microvascular complications to aid in studying their interrelation, and combination therapy. The disease will be...
24 September 2023 2,369 0 View
My study aims at developing an animal model for type II diabetes. I don’t quite understand the power calculation, particularly in regards to the values I would substitute into the equation, as it...
23 September 2023 421 3 View
My research aims to produce a novel mouse model for T2DM and I have two dosage techniques to administer STZ in C57BL/6 mice: single high dose (SHD) and multiple low dose (MLD). Within these...
11 September 2023 671 3 View
I am trying to understand the disparity in dosage of the beta-cell cytotoxic agent streptozotocin, specifically for the induction of type 2 diabetes, between rat models and mouse models. We are...
19 March 2023 606 5 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View
I am developing an ELISA kit for rotavirus A (G1P8 and G1P6). Does anyone know the weight (ng) of these two strains? Or to help me find their weight?
29 July 2024 3,138 2 View