Dominique Liger

3 Questions 2K Answers 0 Followers

Questions related from Dominique Liger

TRP1 deficient yeast strain not growing on 5FAA containing medium?

Hi there, The strain has CEN.PK2-1C background ( MATa ura3-52 trp1-289 leu2-3,112 his3Δ) . I have checked the strain phenotype towards tryptophan: it is unable to grow without tryptophan and...

06 August 2020 3,251 5 View

What is the risk of recombination between plasmids during yeast plasmid shuffling experiment?

Hi, I'm using commercial URA3 and LEU2 plasmids from the same serie for plasmid shuffling in yeast. What is actually the risk of recombination between the two plasmids during the URA plasmid chase...

10 June 2015 9,835 3 View

My S. cerevisiae conditional deletion mutant is dead on FOA but still alive on glucose where it should be dead too! Any idea?

Hi there, I have cloned an essential Sc gene under GAL1 promoter control on a URA3 plasmid (2µ) to generate the conditional genomic deleted strain. As expected, the deleted strain is dead on GAL+...

20 October 2014 4,264 10 View