William Jongmin Kim

4 Questions 10 Answers 0 Followers

Questions related from William Jongmin Kim

How Can I get HIF-1a band in western blotting from CoCl2 induced hypoxia?

I am doing how the hypoxia affect tight junction in retinal pigment epithelial cell (RPE), especially, breaking the RPE layer by hypoxia. I chose to use CoCl2 for inducing hypoxia and performed...

12 March 2017 9,444 7 View

Does anyone do CoCl2 induced hypoxia on retinal pigment epithelial?

I am making hypoxia situation in human pigment epithelial (RPE)using CoCl2. I read the some paper that the cobalt could make hypoxia situation and reduce the cell viability. however i used from...

18 January 2017 5,170 4 View

How disassemble or detach antibody and extracellular vesicle?

We will trying ti isolate marker positive extracellualr vesicle(EV), from pre-enriched EV sample using antibody conjugated bead. The used bead for antibody conjugation is aldehyde/sulfate or...

27 June 2016 5,101 3 View

Can I use protein fragment (Not full length) as standard of ELISA?

Hi I am trying to developing indirect ELISA using antibody and recombinant protein (still finding). For protein stadndard I found some recombinant protein product from variety manufacture, but I...

20 January 2016 2,267 7 View