When I autoclaved the tips that will be used in my DNA and RNA extractions, the tips had drops of water inside, although I covered the boxes of tips with foil.
Just put the tips in an oven at 70C for an hour to dry. They will be okay to use. Better still, buy sterile filter tips so that nucleases in your pipetter don't get into your RNA samples due to aerosols.
Dear Asma,
If not able to use the disposable tips then put the tips in air tight container and covered it with some papers (like newspaper), then the chance of moisture to tips is minimum during autolclaving. But always try to use every thing moisture free for RNA extraction.
Yes... you can use but it matters when u are working with the microliters (less volume) especially in molecular biology techniques. After autoclave keep the tips in oven at 50-60oC for 2hrs till the water gets evoporate then use for the experimentation.
all the best
If need to reuse tips after autoclave, give a post vacuum cycle immediate to autoclave cycle.
If it is also not possible, as richard said, give a air dry in same sterile condition(in most possible ways).
After doing it so, you can use them but there'll be increased fluid retention and unclear appearance though
Don't use the tips when wet at any cost. RNases (enzymes which degrade RNA) could be present. Dry in an oven for a few hours before use.
HI Asma
I agree with Richared, and I think he give you the best answer. And also I suggest you; do this step always for autoclaved equipment, but be aware about oven temperature.
Good luck
although i don't have the experience of using autoclaved tips after drying in oven .. i would suggest you ....try to avoid such type of practice especially for RNA extraction and for rare sample .
if you have enough sample try to perform and share results with us ... lets see what happens....
all the best
I agree with the above, put tips boxes in oven at 70 C for 2 hrs or more till they are dry. This is what we do at our lab.
all the best
hej Asma,
please put the tips in an oven by 70-80°C to dry.
Just imagine if there`s a liquid left in the tip, this would disturbe and affect your pipeted volume.
Best regards
Servus
Christine
For RNA work it is preferred to treat your plastic ware in 1% DEPC for 2 hours at 37 0c by completely submerging them. Then autoclave them for 30 min to inactivate DEPC and then oven dry them at 50 to 60 0c until completely dry. Use polypropylene material tips.
Dear Asma,
You can put the tips in an oven at 70°C to dry. It will take about 1-2 hours.
Bests,
Hovsep
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