Manchala Nageswar Reddy

9 Questions 114 Answers 0 Followers

Questions related from Manchala Nageswar Reddy

Can we use quantitative gene expression levels without reference dye in qPCR MASTER MIX?

Hello I had a small question regarding the reference dye that incorporated in qPCR master mix of 2x Syber green or Taq Man mix. Generally these reference dyes such as ROX is incorporated for the...

08 August 2018 1,091 0 View

Did anyone work to make a Panther analysis for upregulated genes ?

Here I try to make out Panther analysis for highly expressed genes detected in Realtime PCR analysis? will it be possible? or any method can be suggested to make gene clustering? Thanks in advance.

01 January 2017 5,834 1 View

Whether to maintain RNA or Cdna concentration constant with control to measure gene expression level in Real time qPCR.?

Hello I am working on the real time PCR for quantifying gene expression levels. Since the conversion rate of RNA to cDNA depends on reverse transcriptase efficiency which things to be consider for...

12 December 2016 4,502 8 View

Why deep freeze will generate more heat during the initial installation?

Recently we installed the freeze (-20 degree) in our lab. we connected with the power point and run for the test trial.When we are touching the side surface we feel very hot. Please let me know...

01 January 2015 9,565 0 View

Has anyone worked on measuring autophagy induced genes by realtime PCR?

I am trying to see the autophagy levels by inducing with the Rapamycin in a mammalian cells and measure the gene levels by Real time PCR. Has anyone worked with this concept? I need your...

01 January 2015 3,381 9 View

Will it be possible to see the mutational changes in the dengue viral NS 1 construct by site directed mutagenesis?

I am trying to see the role of DENGUE NS1 trough PCR based mutagenesis approach by mutating some of the gene elements responsible for secretion of the protein into the blood stream. Is it...

12 December 2014 4,865 3 View

Will the background caused by mammalian cell lysate matter in ELISA?

Can you tell me whether the background caused by cell lysate matters in giving the signal? wil it be okay if I take lysate cell supernatant after short centrifugation?

11 November 2014 1,455 3 View

Has anyone found the expression of a protein of interest in the mammalian cell lysate by ELISA?

I want to see the protein expression in which the corresponding gene is cloned in a mammalian vector without a secretory signal. I want to see its protein expression in ELISA by using the lysate...

11 November 2014 6,937 5 View

Has anyone used autophagy dye from Enzo Life Sciences?

I am trying to see the autophagy induction in Rapamycin-treated cells by adding autophagy dye. Can anyone suggest the expected RFU, emission and excitation wavelengths?

11 November 2014 8,287 1 View