24 Questions 71 Answers 0 Followers
Questions related from Hayder Obayes Hashim
Hello researchers I have a fresh oral tissue samples collected by Rovers Orcellex Brush , unfortunately I can not use any kit. Please, I need trusted protocol to extract DNA from this brush...
11 November 2019 8,424 3 View
Hello every one I'm working on micRNA expression and need the SNORD44 for normalization , please i need the sequences of SNORD44 primer because i cant find it in the published papers...
05 May 2019 4,889 1 View
Hello everyone I'm working on haplotypes case control association study, for data analysis , I employed two online analysis softwares , The first is (SNPstats) which gave me extremely large odd...
12 December 2018 1,447 1 View
Hello researchers all of us know, that we need a special protocol to extract DNA from frozen blood other than protocol which used to extract DNA from fresh blood . the question is : .what are...
09 September 2018 426 5 View
Hello researchers Can somebody please , provide me the recipes to prepare buffers that used with silica spin column and the protocol to isolate the genomic DNA from blood . best regards
03 March 2018 1,244 4 View
Hello researchers . one of my colleagues develops a new drug and he think that this drug inhibit the activity of RNA polymerase II (transcription ) . the question is , how to estimate this...
02 February 2018 7,692 2 View
Hello researches I'm planning to sequence about 800 bp region of a certain gene to assess its association with a certain disease. the question is , do i have to choose an intronc or exonic...
01 January 2018 3,457 5 View
Hello researchersthere are several reasons to observe a negative peak in HPLC/UV chromatogram , but can this negative peak be due to the presence of fluorescent material. best regards ....
09 September 2017 6,543 4 View
Hello researchers the annealing temperature gradient is the routine step in PCR optimization , but why we do not use annealing time for optimization? regards .
08 August 2017 6,065 15 View
Hello researchers i'm aiming for sequencing 880 nt RNA segment. can i use the ordinary Taq-PCR master mix or i have to use the high fidelity master mix .thank for any suggestions.
07 July 2017 3,857 9 View
Hello researchers I just want to know what happen to the proteins molecules after they migrated out of the gel ( in SDS-PAGE) , of course the simple answer says that the protein migrated to the...
12 December 2016 5,954 11 View
i just wondered , is there any database for compounds UV spectra ? . and can i find a software that simulate the UV spectra for compounds?.Regards
11 November 2016 7,428 1 View
Hello researcherI'm trying to develop a new method to determine the global DNA methylation , and now I'm going to test this method , I'm looking for, if there is a certain organism that have a...
10 October 2016 8,058 2 View
Hello researchersI have an HPLC-size exclusion column , but i don't know its fractionation range . is there any simple procedure to determine the fractionation range of this column?thank a lot .
08 August 2016 8,924 10 View
Hello researchersduring my work on electrophoresis of a certain protein sources , i recognized a good source of positively charged protein .is there any potential application of this proteins ?...
05 May 2016 2,194 4 View
Hello researchersBoth of coating and low vacuum can be used to observe non conductive samples by SEM , but which one is better for image quality ?I will appreciate any comparisonregards
05 May 2016 7,368 8 View
Hello every oneRecently in my lab, I'm working on scanning electron microscope (Quanta 450, FEI), i imaged many ready samples , but i have no idea how to prepare nanotube and nano particles powder...
02 February 2016 572 5 View
Hello everyoneplease i want to find an alloy which can be used as standard for EDX analysis , but for several reasons i can not purchase this alloy from trusted source . so i need your advice for...
02 February 2016 501 1 View
hello everyoneI just wondered , what is the theory behind the ability of different SEM detector to collect and detect a specific type of electrons .for example: there is a secondary electron...
02 February 2016 1,011 5 View
Hello every one I need to quantify EtBr in aqueous solution with expected concentration lesser than 0.5 microgram/ml. I have a UV/HPLC in my lab but I can not find a trusted protocol to perform...
10 October 2015 8,692 4 View
what is the formal way to reference or cite an information from human genome assembly GRCH38 or other assemblies in a paper or review thanks
02 February 2015 7,545 0 View
I extracted DNA from several hundreds blood sample by promega kit , one sample among all the others show a two clear fluorescent bands migrate to the negative pole , those bands appear without any...
01 January 2015 1,886 14 View
: Hello every one Please : can anyone guide me to a trusted and free software for genetic association data analysis and statistics . thank .
12 December 2014 8,146 4 View
Hi everybody : Please : Can anybody tell me how long can I store the PCR primer solution under 20C ? Are there any additives or methods which can improve the primer stability during the storage ?
10 October 2014 5,693 16 View
