What is the method in detail of reporter gene of luciferase assay ?

I will do the reporter gene by luciferase assay to study the promoter activity?i will use pgl3 basic vector and prl-tk,, the cell line MCF-7and MCF-7 , I will use lipofectamine 2000 and optimum...

04 May 2015 8,038 3 View

What is benefit added of BSA with digestion buffer ?

I have two restriction enzyme XhoI and HindIII , i used BSA only with digest vector not interested DNA , is it ok ?

04 May 2015 2,818 2 View

Does gene expression of any gene in breast cancer line reflect the gene expression in vivo in the breast ?

when we dealing the cell to convert to cell line , the property of the cell line like gene expression of cell or phenotype from the vivo tissue ? do we can depend the result expression of gene in...

02 March 2015 2,360 9 View

Why did I not got any results with lkb1 primer in real time pcr?

I had mcf-10 cell and was grown in dmem/f12 media with fbs and AB and rna and dna was extracted from the cell ,, when I did RT-PCR with cDNA , I got results with GAPDH but not with my interested...

31 December 2014 9,018 3 View

Why the different size of band with the same gene in pcr ?

31 December 2014 6,189 8 View

What are the reasons for low expression for the gene LKB1 in a mcf-10 cell line?

31 December 2014 8,928 6 View

Can I use pipette tips that have water in them after autoclaving in RNA extraction?

When I autoclaved the tips that will be used in my DNA and RNA extractions, the tips had drops of water inside, although I covered the boxes of tips with foil.

11 December 2014 7,777 13 View

If I isolate DNA and RNA from cell monolayer culture by Trizol, do I need to centrifuge after adding Trizol and before adding choloroform?

11 December 2014 2,515 5 View

What is appropriate media for MCF-7 cell and MCF-10 ?

10 November 2014 1,972 1 View

What is the condition media from cell culture and what are the advantages in using it?

In my experiment, I collected condition media from breast cancer cell culture MDA-231 and I added it to normal breast cell to convert it to cancer, what is the advantage when we used condition...

09 October 2014 8,541 6 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View