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I will do the reporter gene by luciferase assay to study the promoter activity?i will use pgl3 basic vector and prl-tk,, the cell line MCF-7and MCF-7 , I will use lipofectamine 2000 and optimum...
04 May 2015 8,101 3 View
I have two restriction enzyme XhoI and HindIII , i used BSA only with digest vector not interested DNA , is it ok ?
04 May 2015 2,869 2 View
when we dealing the cell to convert to cell line , the property of the cell line like gene expression of cell or phenotype from the vivo tissue ? do we can depend the result expression of gene in...
02 March 2015 2,410 9 View
I had mcf-10 cell and was grown in dmem/f12 media with fbs and AB and rna and dna was extracted from the cell ,, when I did RT-PCR with cDNA , I got results with GAPDH but not with my interested...
31 December 2014 9,071 3 View
I have rna for MCF-10 and MCF-7 ....the concentration of rna of mcf-7 is 884 ng/microliter with 260/280 1.76 .. the concentration of rna of mcf-10 is 668 ng/microliter with 260/280 1.91 I convert...
31 December 2014 6,245 8 View
The MCF-10 cell line was grown with DMEM12, 10% serum, 5% antibiotic and HuMEC supplemented. When we studied gene expression of LKB1 by RT-pcr ROCHE the results is small in triplicate (CT 35 ,42...
31 December 2014 8,991 6 View
When I autoclaved the tips that will be used in my DNA and RNA extractions, the tips had drops of water inside, although I covered the boxes of tips with foil.
11 December 2014 7,833 13 View
My cells are MCF-7 and MCF-10. I want extract DNA and RNA from these cell using Trizol. Do I add Trizol directly into the flask after removing the media? What amount of Trizol is needed if I grow...
11 December 2014 2,579 5 View
I have mcf-7 and mcf-10 and I grow them in DMEM 12 media. Is this a suitable method?
10 November 2014 2,020 1 View
In my experiment, I collected condition media from breast cancer cell culture MDA-231 and I added it to normal breast cell to convert it to cancer, what is the advantage when we used condition...
09 October 2014 8,648 6 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...
03 August 2024 9,918 0 View
Dear All I would like to know the minimum number of animals (rats) to be studied for a pilot/preliminary research on intradermal drug delivery. Can I proceed with 1 animal per group for a pilot work?
02 August 2024 4,570 1 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View
Hello! I have this scale which had 10 items initially. I had to remove items 8 and 10 because they correlated negatively with the scale, and then I removed item 9 because Cronbach's alpha and...
01 August 2024 4,606 7 View
In my experiment, I treated RAW macrophages with 100 ng/mL of LPS. After 24 hours, I collected the supernatant and replaced it with fresh media. I then quantified the cytokine levels of TNF and...
01 August 2024 476 2 View
I've seen articles that primarily focus on alpha and beta activity in the frontal regions, but these studies often compare healthy subjects with those having various pathologies. I haven't seen a...
31 July 2024 7,259 1 View
