Prakash Chandra

4 Questions 144 Answers 0 Followers

Questions related from Prakash Chandra

Regarding to expression of specific gene ?

I want to know expression level of a certain genes I do have primers . I have few questions: 1. is it necessary for me to quantify RNA? if it is only 1 sample? 2. Is it necessary that i should...

04 April 2017 6,419 4 View

Regarding the establishment of Cell lines.

As said above I have some questions on Establishment of Cell line: as I am willing to establish and characterize a cell line 1. let me know what are the basic requirements and equipment are...

10 October 2015 6,264 4 View

Can anoyone help with a problem in the 5'RACE?

I am working on molecular cloning of a gene we have already synthesized the 3'end of CDS now we want to clone 5' end and for the same I am following 5'RACE strategy. In final step with PCR anchor...

05 May 2014 5,603 8 View

We used to take O.D at 600 nm for s culture. Could somebody tell me why we use mostly 600 nm and why not more or less?

I know if we use UV range it will harm the cells.

04 April 2014 3,072 8 View