Suheir Nassar

8 Questions 23 Answers 0 Followers

Questions related from Suheir Nassar

How can I explain the Ct values in my qPCR standard curve for B.bifidium?

the qPCR standard curve for a B.bifidium DNA starting from 20ng to 0.00002ng is giving strange Ct values , eventhough i repeated the qPCR three times in case i mixed up the serial dilution tubes....

03 March 2015 9,387 8 View

How can I convert the DNA amount into CFU/gram feces in qPCR quantification of bacteria in stool samples?

I understood that to generate a standard curve for a certain bacteria, the Ct values should be plotted relative to corresponding serial 10-fold dilutions of template DNA of known concentration...

10 October 2014 1,962 8 View

In DNA isolation from Stool using zirconia beads, would vortexing the samples be an alternative of using a bead beater?

I am using the QIAGEN kit for DNA isolation from stool for bacteria detection. I am also using Zirconia beads for mechanical destruction of the bacterial cells as a step in DNA isolation. I don't...

10 October 2014 5,196 8 View

Can anyone help with reference gene selection for qPCR gene expression analysis?

I am going to test the gene expression of IL-33 , Hinf I and other immune response related genes in blood. I am confused about the reference gene that I should select and order primers for along...

09 September 2014 2,116 7 View

How do you deal with Bacterial DNA degradation and get a PCR product?

DNA was extracted from three anaerobic Gram positive bacteria, (C.difficile,B.bifidium and B.lactis) using a QIAGEN kit. PCR was performed for 16S rDNA segment specific for each one. Only B.lactis...

09 September 2014 8,202 2 View

Which TRI reagent is preferred for use in RNA isolation?

I'd like to ask if there is difference in TRI reagent supplied by several companies: Sigma, Peqlab, invitrogen...etc. Is there any one preferred for use for RNA isolation?

04 April 2014 3,923 10 View

RNA isolation using TRI reagent: Can i use chloroform that is stabilized with ethanol?

In RNA isolation (from frozen blood samples) using TRI reagent , can i use chloroform that is stabilized with ethanol?

04 April 2014 874 6 View

Can anyone help with DNA extraction of bacteria from human stool samples?

I am using QIAamp stool DNA mini kit from QIAGEN -the protocol:stool pathogen detection. However, I've hardly got DNA out of my frozen stool samples (around 4ng/ul) knowing that the kit implies...

04 April 2014 7,764 12 View