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Questions related from Asma Future
I will do the reporter gene by luciferase assay to study the promoter activity?i will use pgl3 basic vector and prl-tk,, the cell line MCF-7and MCF-7 , I will use lipofectamine 2000 and optimum...
05 May 2015 8,072 3 View
I have two restriction enzyme XhoI and HindIII , i used BSA only with digest vector not interested DNA , is it ok ?
05 May 2015 2,855 2 View
when we dealing the cell to convert to cell line , the property of the cell line like gene expression of cell or phenotype from the vivo tissue ? do we can depend the result expression of gene in...
03 March 2015 2,390 9 View
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02 February 2015 8,369 0 View
I have rna for MCF-10 and MCF-7 ....the concentration of rna of mcf-7 is 884 ng/microliter with 260/280 1.76 .. the concentration of rna of mcf-10 is 668 ng/microliter with 260/280 1.91 I convert...
01 January 2015 6,228 8 View
I had mcf-10 cell and was grown in dmem/f12 media with fbs and AB and rna and dna was extracted from the cell ,, when I did RT-PCR with cDNA , I got results with GAPDH but not with my interested...
01 January 2015 9,051 3 View
The MCF-10 cell line was grown with DMEM12, 10% serum, 5% antibiotic and HuMEC supplemented. When we studied gene expression of LKB1 by RT-pcr ROCHE the results is small in triplicate (CT 35 ,42...
01 January 2015 8,964 6 View
My cells are MCF-7 and MCF-10. I want extract DNA and RNA from these cell using Trizol. Do I add Trizol directly into the flask after removing the media? What amount of Trizol is needed if I grow...
12 December 2014 2,553 5 View
When I autoclaved the tips that will be used in my DNA and RNA extractions, the tips had drops of water inside, although I covered the boxes of tips with foil.
12 December 2014 7,812 13 View
I have mcf-7 and mcf-10 and I grow them in DMEM 12 media. Is this a suitable method?
11 November 2014 2,003 1 View
In my experiment, I collected condition media from breast cancer cell culture MDA-231 and I added it to normal breast cell to convert it to cancer, what is the advantage when we used condition...
10 October 2014 8,610 6 View
Ct of tumor cell is 28.79 Ct of control cell is 28.83 The gene expression ratio after calculation (livak method ) is 1.96
05 May 2014 7,519 3 View
When I extracted total RNA from cell (tumor and normal) and I made one amount of RNA to convert to cDNA, I got no reverse transcription reaction. I diluted cDNA to 100 microliters, in addition to...
04 April 2014 8,241 3 View
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02 February 2014 5,001 3 View
