What is the method in detail of reporter gene of luciferase assay ?

I will do the reporter gene by luciferase assay to study the promoter activity?i will use pgl3 basic vector and prl-tk,, the cell line MCF-7and MCF-7 , I will use lipofectamine 2000 and optimum...

04 May 2015 8,038 3 View

What is benefit added of BSA with digestion buffer ?

I have two restriction enzyme XhoI and HindIII , i used BSA only with digest vector not interested DNA , is it ok ?

04 May 2015 2,818 2 View

Does gene expression of any gene in breast cancer line reflect the gene expression in vivo in the breast ?

when we dealing the cell to convert to cell line , the property of the cell line like gene expression of cell or phenotype from the vivo tissue ? do we can depend the result expression of gene in...

02 March 2015 2,360 9 View

What is the normal level of concentration of TNF-alpha in rat colon tissue ?

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01 February 2015 8,332 0 View

Why did I not got any results with lkb1 primer in real time pcr?

I had mcf-10 cell and was grown in dmem/f12 media with fbs and AB and rna and dna was extracted from the cell ,, when I did RT-PCR with cDNA , I got results with GAPDH but not with my interested...

31 December 2014 9,018 3 View

Why the different size of band with the same gene in pcr ?

31 December 2014 6,189 8 View

What are the reasons for low expression for the gene LKB1 in a mcf-10 cell line?

31 December 2014 8,928 6 View

Can I use pipette tips that have water in them after autoclaving in RNA extraction?

When I autoclaved the tips that will be used in my DNA and RNA extractions, the tips had drops of water inside, although I covered the boxes of tips with foil.

11 December 2014 7,777 13 View

If I isolate DNA and RNA from cell monolayer culture by Trizol, do I need to centrifuge after adding Trizol and before adding choloroform?

11 December 2014 2,515 5 View

What is the condition media from cell culture and what are the advantages in using it?

In my experiment, I collected condition media from breast cancer cell culture MDA-231 and I added it to normal breast cell to convert it to cancer, what is the advantage when we used condition...

09 October 2014 8,541 6 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

No title

02 March 2021 3,060 3 View