Hi,
I would like to ask a simple question. Can we filter the contaminated DMEM with 0.25 um filter paper in order to get it useable again?
Thank you
Hi
Filtration of media in 0.2 micron may remove the contaminants (particles). But the microbial waste/toxin (it wont filtered out) will impede the growth of cells.
Better discard this media and use fresh one.
Hi
Filtration of media in 0.2 micron may remove the contaminants (particles). But the microbial waste/toxin (it wont filtered out) will impede the growth of cells.
Better discard this media and use fresh one.
Dear Samihah,
I agree with Karthikeyan,
Because of contaminated DMEM contains toxin and by product of bacteria and fungi, so, it is preferable not use it. Filtration of the media can not remove toxin and by product.
Thank you for making the decision is easy to be concluded. My DMEM was not contaminated, I just prepare for the possibility of contamination.
Dear Samihah,
As a general rule, think about the cost of the contaminated reagent. If it is something cheap, such as media, throw it out as soon as you realise that something is wrong with it. The potential unwanted consequences of using it, especially lost time, are not worth it, not to mention that the cost of the filter is probably not that different to the replacement cost of the media. If it is something rare, unique, expensive or time consuming to replace, then I would consider ways to save it, but always as a last resort.
Thea
Just in case you got contamination around 1-2 days, after filtering the media, cells will grow well no problem. For the experiment to observe cell localization, it will not be affected. However, as Thea Anna Monks said we dont know what will happen for long term period. In my opinion, the media can be used but we should throw the cell after several passages.
To reduce the probability of Medium's contamination and if you don't need high amount of medium every time of feeding!
you can fill 50 ml "falcon" and feed from it instead of the main flask!
Don't waste time filtering it- throw it away and start again. What if the contaminant is not caught by the size of the filter.
Thank you for all suggestions. The method for separating media in 50ml tube have been applied by me. For good outcomes, I will just discard the medium. Grateful, the media that I have prepared for 1 week, is still ok and not contaminated.
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