Trang Nguyen

9 Questions 56 Answers 0 Followers

Questions related from Trang Nguyen

The DNA concentration of Flag-C1 (control) and Flag-VAV2 should be different when transfect into the cell?

I am doing western blot to detect the activation of Rac1 (Rac1-GTP). VAV2 is known to activate Rac1. I want to confirm by western blot. The length of Flag-C1 (control) is shorter than Flag-VAV2...

01 January 2016 4,963 1 View

Why can I only detect the band of anti-GFP-Ab in GFP-N1 but not in TIAM1-GFP and VAV2-GFP of transfected cells?

I do the western blot to check the anti-GFP antibody. I transfected the cells with GFP-N1, TIAM1-GFP and VAV2-GFP in HEK293T cell line. When I check the fluorescence microscope, I can detect GFP...

05 May 2015 4,357 6 View

Why does the mRNA level of control siRNA is lower than the target siRNA?

I do realtime PCR for a target protein after doing siRNA transfection. However, every time when I run real time PCR, the mRNA level of target protein is higher than in the control siRNA. In the...

04 April 2015 7,129 10 View

What is the difference between MG132 proteasome inhibitor and protease inhibitor cocktail tablets?

I could not detect my target endogenous protein but I can detect over-expressed protein. I used Protease Inhibitor Cocktail Tablets. My friend told me that my target protein could have a high turn...

04 April 2015 566 5 View

Why couldn't the antibody recognize the endogenous protein?

I want to test the protein expression of a protein around 130kD but I could not detect it. When I over-expressed the protein, I can detect the protein but not the endogenous protein. I tested...

04 April 2015 2,753 17 View

How many ways are there to make siRNA?

I intend to make siRNA for my protein. I intend to send for the company to synthesis 3 difference region in the gene. Each region will be around 21 nucleotides. Then I will combine 3 single...

01 January 2015 9,820 9 View

Can I generate siRNA that can knock down all of the family of RGL (RGL1, RGL2, and RGL3)?

I found that over-expressed RGL3 can induce cell migration. I bought siRNA RGL3 and transfected to NIH3T3 cell line. Then, I observe cell migration of depleted cell but I could not observe a...

01 January 2015 6,984 8 View

How can we conclude one protein can enhance cell migration?

I over-expressed RGL1 in NIH3T3 cell line and measure cell migration. I found over-expressed RGL1 increased cell migration compared to the control cells. I try to knock down the endogenous RGL1 to...

12 December 2014 5,226 10 View

How can I prove GEF interacts with Rac1, cdc42, and RhoA?

I found a new GEF and I want to know which small GTPases will be activated by this GEF. I did FRET biosensor assay, I found this GEF interact with Rac1 and cdc42 but not RhoA. I did another...

12 December 2014 6,859 24 View