16 Questions 27 Answers 0 Followers
Questions related from Samihah Zura Mohd Nani
Hi, I would like to know either i need to perform normality test at first before do the analysis such as ANOVA. My data is regarding effect of drugs. I have 3 replicates for each concentration of...
11 November 2016 8,335 10 View
As I know, the reading for the Competitive ELISA assay will be inverse with the concentration of standard and sample tested. Thus, how can I interpret or convert the result later as we prefer to...
02 February 2016 4,495 4 View
Hi, Most of the research papers or protocols mentioned that DMEM should be supplemented with 10% FBS or FCS for the cell culture. However, it does not mentioned clearly 10 % in the DMEM or else....
01 January 2016 453 4 View
I have faced several times in my cell culture, of having small particles or dots that have swing moving. Not sure if it bacteria, or not. By the way, It does not has jerking movement. Or it just...
12 December 2015 3,773 4 View
Hi, I had thawed the 3T3-L1 cells from the liquid nitrogen and culture it in the T75 flask. The cells did only attached about 1 % confluence after a week, and most of the cells were still...
12 December 2015 3,997 3 View
Hi, I would like to ask a simple question. Can we filter the contaminated DMEM with 0.25 um filter paper in order to get it useable again? Thank you
12 December 2015 5,585 9 View
Why need different serum for adipogenesis assay?
11 November 2015 9,803 4 View
I have liquid sample and using cell assay to test its effects. My sample does not have solid or powder form, Extraction, freeze drying, or lyophilization of it will deteriorate its quality. Thus,...
11 November 2015 5,883 0 View
I have been understood that bovine and calf are the same species. Thus, what are difference for: 1. Fetal bovine serum 2. Fetal calf serum 3. Bovine serum 4. Bovine calf serum Some research used...
10 October 2015 10,284 0 View
Hi, I use DMEM that was been as complete growth medium (CGM). If working volume for 24 well plate is 1ml, how can I distinguish between how much volume of CGM (grwoth medium) and treatment sample...
09 September 2015 2,526 7 View
How much of MgCl2 required to make the solution of 1000ppm Mg from MgCl2 in 5 ml water? Is it molecular weight should be used in the calculation?
08 August 2015 9,731 6 View
I need to dilute drugs. In my case, I would like to dilute chromium picolinate in the solvent. What is the best solvent can be used either DMSO, acetone, PBS or etc. How can we justify that the...
08 August 2015 5,411 7 View
The working volume for 24 well is 1 ml, I should standardized the medium below 1 ml. For example, I will put complete growth medium (CGM) about 500 ul in each 24 well plate. But when I added the...
07 July 2015 2,314 5 View
I want to test the effects of some nutrient such as Mg in the cell culture. What standard of concentration should I follow? For example if I use a cell line derived from an animal, do I need to...
05 May 2015 2,462 1 View
Does anyone can explain and perhaps give guidelines on how to read the lipid droplets or accumulation in 3T3-L1 cells after Oil Red Oil staining? This is because I cannot confidently calculated...
05 May 2015 3,084 4 View
Hi, anyone knows which animals are suited for a aortic formation study (cardiovascular disease) - rat or rabbits? Rat has a tiny aorta which is difficult to be sampled and cut compared to rabbit.
10 October 2014 6,151 8 View
