Do I need to perform normality test for dose response data?

10 November 2016 8,239 10 View

How can I use the reading for Competitive ELISA analysis which has inversely proportional reading?

01 February 2016 4,409 4 View

Can I know either 10% FBS or FCS should be put into DMEM or treatment working medium?

31 December 2015 190 4 View

How much is the maximum period for 3T3-L1 cells usually take to attach?

Hi, I had thawed the 3T3-L1 cells from the liquid nitrogen and culture it in the T75 flask. The cells did only attached about 1 % confluence after a week, and most of the cells were still...

11 December 2015 3,915 3 View

What is the small particle that has a swinging moving in the media of my cell culture?

11 December 2015 3,626 4 View

Can contaminated DMEM medium be filtered and used again?

11 December 2015 5,477 9 View

Why use fetal calf serum to culture cell and use fetal bovine serum in cell assay?

Why need different serum for adipogenesis assay?

10 November 2015 9,657 4 View

How do I prepare liquid sample or even volume for cell culture assay?

I have liquid sample and using cell assay to test its effects. My sample does not have solid or powder form, Extraction, freeze drying, or lyophilization of it will deteriorate its quality. Thus,...

10 November 2015 5,815 0 View

What is the suitable working volume, DMEM, vs. sample solution?

08 September 2015 2,356 7 View

What is the best solvent for drugs?

I need to dilute drugs. In my case, I would like to dilute chromium picolinate in the solvent. What is the best solvent can be used either DMSO, acetone, PBS or etc. How can we justify that the...

07 August 2015 5,328 7 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

No title

02 March 2021 3,060 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

No title

28 February 2021 9,936 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View