As I know, the reading for the Competitive ELISA assay will be inverse with the concentration of standard and sample tested. Thus, how can I interpret or convert the result later as we prefer to have the increase or positive reading?
If there is a decrease of the signal with increasing concentations of your competitor (standard competitor) you can just interpolate the OD values of your samples with respect to such a curve. It doesnt matter that the correlation is inverse. That is if you want to calculate the concentrations of competitor analyte in your samples.
I have interpolate the data, the final value of interpolated concentration is decreased compare to its actual value which is increased before the data been fit with standard curve. When I make a graph using Interpolated X mean value, the graph show an increase of absorbance with the increase of concentration. It is my graph is correct? It seem logic right now.
I dont understand what you did. What is the goal of your experiment? Are you calculating the concentrations of your competitor anlyte or not?
The goal is to know the difference between control and treatment group.
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