Vaccines

A forum for discussion on vaccine development, delivery, manufacturing, research, and formulation. | Review and cite VACCINES protocol, troubleshooting and other methodology information | Contact experts in VACCINES to get answers

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Questions related to Vaccines

How should one teach ETHICS to the new generation of college students in 2024?

More and more, teaching ETHICS has become an important part of the college curriculum, but teaching it has not always been easy or up to part with the generational changes in the student...

03 January 2024 7,776 6 View

Is it a different method for dilution of vaccine with adjuvant?

in the NIH test for checking the potency of the rabies vaccine, we must make dilutions. In the second dilution, we have a sharp drop and the mortality of mice. I wondering if my dilution technique...

09 December 2023 4,443 2 View

How to do the transfection by lipofectamine method? at what ration we will add lipo:dna?

lipofectamine method for the transfection in MDA-MB231 cell line

19 October 2023 3,226 0 View

Could ER stress be the mechanism that triggers inflammation in rheumatological patients?

I am open to collaboration with your research on rheumatic diseases and ER stress. Kind regards

19 September 2023 6,583 0 View

Does Aprotinin contain a Heterocyclic compound with Nitrogen in Chemical structure?

Aprotinin from Bovine lung consists of 58 amino acid residues that are arranged in a single polypeptide chain, cross-linked by three disulfide bridges. Aprotinin is a single chain polypeptide...

18 June 2023 639 3 View

Name the techniques that is used for the estimation of MPN of coliforms in a water sample?

Durham vials, Sophisticated microscope, Water bath, Inoculation loops,Fermentation tubes.

31 May 2023 6,865 0 View

Are researchers and pharmaceutical industry specialists aware of people noticing positive side effects from COVID-19 vaccines?

Such positive side effects may include, but are not limited to less overall pain, including arthritis pain.

20 May 2023 7,002 0 View

How to carry out selective hydrogenation of vanillin to vanillyl alcohol using Ni-Cu catalyst?

I have experimented with various methodologies, but I am getting p-cresol in end instead of vanillyl alcohol. Can anyone suggest any suitable parameters?

27 March 2023 6,240 2 View

Use of Oncept melanoma vaccine in 69 canine oral malignant melanomas in the UK by S. Verganti- number of dogs who received the induction course?

This paper states that all dogs received it but then states that 76.8% received 4 doses (which is the induction course)??

17 March 2023 4,295 0 View

Any incidences of allergic reaction due consuming of to green tea? What could be the possible allergen specific to green tea?

What could be the possible allergen in case of Allergic stomatitis to green tea?

22 November 2022 2,912 1 View

Can delayed reactions to allergen immunotherapy/shots be an indicator of H4 receptor dysfunction?

Asking for myself so I can figure out what to ask doctors about after my allergist decided that my situation was beyond his scope and I wait several months for my appointment with the immunologist...

19 November 2022 3,683 0 View

How to link an adjuvant with a vaccine construct using reverse vaccinology approach?

Is it necessary to link an adjuvant to a multiepitope vaccine construct? If necessary, how we can find its sequence?. I want to add Montanide or Freund's adjuvant to vaccine construct but confused...

05 August 2022 1,330 0 View

Is there a Guidelines for the Euthanasia of Animals in Libya ?

If am proceeding an experiment and I want to follow the Euthanasia of Lab Animals after finishing my experiment. Is there a Libyan guideline for that or I should follow the AVMA Guideline or NIH ?

04 August 2022 9,714 0 View

How can heat stress genes Act in poultry?

High ambient temperatures are a critical challenge in the poultry industry which is a key producer of animal-based food. To evaluate heat stress levels, various parameters have been used,...

14 March 2022 6,032 9 View

How to estimate the pause days in groups of laying hens reared on deep litter floor system?

I have study and planned to estimate the pause days for each group of laying hens. this laying hens kept on deep litter floor system

20 September 2021 2,673 6 View

What is the model antigen to study DC based CLL vaccines?

What is the best model antigen to study the effect of engineered DC based vaccines in Chronic lymphocytic leukemia. In solid tumors people usually use ovalbumin for this purpose but is it also...

10 September 2021 6,804 0 View

Do we suggest Post exposure prophylaxis for rabies to a calf??

If a calf say of 7 days old got bitten by a rabid suspected dog then what should be the course of action except euthanasia?

26 August 2021 1,825 4 View

What are the simple methods of inducing heat stress in broilers under deep litters system?

I am currently working on the use of phytogenics substances and non-synthetic materials in controlling heat stress in broilers in tropical areas.

20 July 2021 9,826 8 View

Is there any protein tags (myc, flag, v5, ha, etc.) works as an adjuvant to enhance the protein based vaccine immunity?

Plan to add a tag with the GOI for the protein-based vaccine. Are there any tags or certain short AAs that can activate the innate immune response?

02 June 2021 5,201 2 View

What the effects of heat stress on the antioxidant enzymes in poultry?

In Egypt, ambient temperature can remain consistently high for extended periods of time and sudden recurrent hot and humid waves that have more harmful effects. So poultry production suffers...

30 May 2021 2,812 6 View

With a valid reference, why there is currently no licensed vaccine for brucellosis in humans?

Despite the induction of both humoral and cellular immune responses by different vaccine candidate, what are the limitations for the development of the brucellosis vaccine for humans which none of...

27 May 2021 7,366 5 View

Is COVID VACCINE development and storage possible at Room Temperature?

Indian scientists have developed a candidate vaccine against the coronavirus disease that remains active after long-term storage at room temperature, raising hopes for novel vaccines that would...

12 May 2021 3,087 6 View

Anti-Covid 19 NASAL Vaccine: what are the present stage of these vaccines ? What will be the possible benefits?

Anti -Covid 19 NASAL Vaccines are under trial - as per newspaper reports. What are the different types (techniques used) of such vaccines under study at different laboratories? What are the...

21 April 2021 9,135 10 View

Is it mandatory to wear the mask even while struggling to breathe and die eventually ?

In a video, I found a lady struggling to breathe at the entrance of the hospital and put on hold due to lack of availability of beds. She was apparently "Paradoxically breathing" with increased...

19 April 2021 6,537 0 View

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