Can an Alexa Fluor 488 conjugation kit be used to differentiate or only target and fluroecse c-Fos Rabbit mAb while leaving HA unaffected?

03 December 2024 0 4K Report

There was an accidental mixing of HA and c-Fos Rabbit mAb into the same vile. We are trying to stain for only c-Fos. However, both HA and c-Fos Abs were raised in rabbit. So the Alexa Fluor 488 will bind to both of the primaries. I read about an Alexa Fluor 488 conjugation kit but found limited information. Has anyone ever used one of these and knows if it can be used to help fluoresce our c-Fos Rabbit mAb and not the HA?

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