in the NIH test for checking the potency of the rabies vaccine, we must make dilutions. In the second dilution, we have a sharp drop and the mortality of mice. I wondering if my dilution technique is incorrect. I use a sampler for dilution. is it a different method for dilution of vaccine with adjuvant?
Abhishek Rajput
It sounds like the sudden increase in mortality during the second dilution could be because of how you’re diluting the vaccine. Using a sampler might not give you the precision you need. Try using a pipette for more accurate measurements. And when dealing with vaccines that have adjuvants, make sure to follow the recommended methods carefully to keep the vaccine potent and safe for use.
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