Is there any method I can use to isolate single cells (i.e cardiomyocytes and non-cardiomyocytes) from snap from rat heart tissue?

I have GD18.5 heart tissue currently snap frozen in -80, apart from mRNA and protein analysis on whole tissue, is there a way of isolating single cell populations to study localization of a...

19 January 2021 4,044 3 View

How to stop sperm cells clumping during fixation?

I've been optimising a flow cytometry protocol to assess intracellular proteins in human sperm. Most published methods describe fixing with 4%PFA after washing and pelleting the cells. I am...

06 September 2020 2,914 2 View

Help with human nuclear DNA primers for qPCR?

03 January 2019 6,565 3 View

How to summarise systematic review findings in a rapid review?

22 February 2017 6,471 5 View

Does anyone have a protocol for immunostaining in E. coli?

I am looking to ideally fix E. coli to a slide, stain with an anti-his or anti-flag type antibody, follow with a fluorescent secondary and image. Does anyone have an established protocol? -Thanks-

13 August 2014 9,393 1 View

SDS page software for analysis?

I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...

02 March 2021 6,270 3 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

Is the sinovac vaccine safe and effective for people over the age of 60 ?

Dear respective researchers, I was asked by my institution to be vaccinated this week using the sinovac vaccine. However, from some of the information I got, this vaccine is not recommended for...

01 March 2021 7,781 6 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

If a given country passes mandatory 'vaccine passport rule' to go outdoors, otherwise stay at home, can it help to vaccinate all against COVID-19?

Wether vaccine passport declared not to go out of home without it may help taking COVID-19 vaccine. Can't it break individual's right to protect the community? Your comments on perspectives...

27 February 2021 5,549 23 View

How long do vaccines protect us against COVID 19?

Or will we have to get vaccinated every year, as in the case of influenza?

27 February 2021 1,048 5 View

Any Differences in COVID-19 vaccines?

Are there any differences (outside of the clinical trials that led to their EUA) observed in the rate of infection among those who have received the Pfizer vs Moderna vaccine since the mass...

22 February 2021 9,727 6 View

Can COVID-19 vaccines protect against the emerging strains of SARS-CoV-2?

Can COVID-19 vaccines protect against the emerging strains of SARS-CoV-2?

18 February 2021 8,221 8 View

Would different strains of SARS-CoV-2 required different vaccines shots?

Would different strains of SARS-CoV-2 required different vaccines shots?

18 February 2021 6,934 6 View