This paper states that all dogs received it but then states that 76.8% received 4 doses (which is the induction course)??
I have 2 groups of students, both groups have pre-post qual and quant questions following a teaching intervention. The students have 4 clinical skills sessions taught by academic staff, 2 sessions...
31 December 2021 929 4 View
I was wondering if it was possible to use the 'Norm nm' and 'Norm Abs' measurements from a nanodrop to determine the concentration of nanoparticles in solution, especially when mixed with other...
08 September 2021 6,409 2 View
I wonder if there are any jurisdictions in the world that do not provide an appellate body for civil disputes (in the sense of a legal and, albeit limited, factual review of the first instance...
20 January 2021 7,762 14 View
I have GD18.5 heart tissue currently snap frozen in -80, apart from mRNA and protein analysis on whole tissue, is there a way of isolating single cell populations to study localization of a...
19 January 2021 4,219 3 View
I've been optimising a flow cytometry protocol to assess intracellular proteins in human sperm. Most published methods describe fixing with 4%PFA after washing and pelleting the cells. I am...
06 September 2020 3,070 2 View
As the Schistosoma infections may lead to many serious conditions, safe and effective medication is needed
11 March 2020 1,782 6 View
I'm working with the cell line CRL-1446, which should beat in culture with the addition of acetylcholine (according to ATCC). We culture in 1% FBS DMEM to form myotubules as suggested by ATCC....
10 November 2019 4,506 2 View
Hi, I have been using ImageJ to measure the area of fetal ovaries, with Cleaved Caspase 3 and mvh/ddx4 expression for my IF. mvh is the green channel and CC3 is on the red channel. I haven't had a...
24 June 2019 3,759 3 View
I am wanting to look at the ratio of nuclear vs mitochondrial DNA in the human cell line MCF7 cells Using qPCR (syber green and a rotogene machine using a total cell pellet DNA). I’ve designed and...
03 January 2019 6,684 3 View
I'm doing a protein precipitation protocol using TCA (trichloroacetic acid) for the first time. My protocol says to dissolve 500g TCA into 350ml dH20 (and store at room temp) for a 100% (w/v)...
07 November 2018 7,475 0 View
I am conducting my research regarding blood coagulation in patients with CKD. I observed a reduction in post hemodialysis aPTT and PT values compared to pre hemodialysis values. But most of...
30 July 2024 3,467 0 View
Background: I am developing an RdRp assay for Dengue virus-3 and need to express and isolate the NS5 protein's RdRp domain (~101 kDa). My expression vector includes a GST tag followed by a 6x His...
13 July 2024 9,950 3 View
I have been looking at papers for protein expression and trying to follow the process. I'm using TB medium to do the process, and the paper says to grow to an OD600 = 1.8 at 37 degrees, then let...
30 June 2024 6,501 5 View
I am trying to do Grounded Theory in documentary research. I have obtained the most frequent terms (induction) to perform deductive coding and so, articulate a hypothesis about the hidden...
05 June 2024 2,599 2 View
Hello. I'm currently working on a control system for a doubly fed induction generator (DFIG) as part of my thesis project. Traditionally, fuzzy logic controllers (FLCs) use the error (e) and the...
27 May 2024 1,732 2 View
Hi, our lab has recently started working with primary hepatocytes in 3D form. The lot we are using has high basal activity which declines as the spheroids age. We are looking to undertake drug...
21 May 2024 1,320 0 View
procedure in which both the axial portion of the alar wing and all of the alar fold are removed by amputation. The procedure results in a very cosmetic appearance when done properly.
16 May 2024 9,828 0 View
I am planning to do invitro transcription and capping for an mRNA vaccine generation. Can anyone suggest whether it should be done on a bench top or inside a laminar hood?
14 May 2024 186 3 View
For the design of three phase inverter for three phase motor drive, which is better using low DC voltage (12 V or 24 V DC) for the inverter stage then step it up using three phase transformer, or...
08 May 2024 3,491 3 View
Frankly speaking thrice time affected by COVID although covaxin taken but no harm to cancer patients.it depending upon metastatic position at that time of COVID affected person s . I was patient...
02 May 2024 1,818 3 View