Please suggest latest published standards on museum collection storage & maintenance. Has COVID brought any significant changes in the protocol?

What is the level of compliance to these published standards in textile museums and what is the impact of this compliance on museum budget and infrastructure?

28 December 2020 757 2 View

Problem associated with glyco protein in SDS-PAGE ?

The membrane glycoprotein which is made up of 79% lipids and 20% protein whenever we go for SDS PAGE with fresh sample will give clear profile in some samples and not with other samples....

08 May 2019 5,657 3 View

Shall we publish our peer-reviewed article in research outreach (https://researchoutreach.org/)?

I would like to know about the research outreach (https://researchoutreach.org/) publishing agency. For my peer-reviewed article, I am getting an offer to republish that in this. However, I can...

24 January 2019 9,027 88 View

How calculate the percentage of sporulation of different bacteria with microplate method?

I have just launched a test of sporulation of three different bacteria with several culture media in a shake flask experiments. I've recently consulted a publication entitled: "Enhanced Spore...

16 July 2017 9,936 23 View

Does anyone have any experience with Protein transfection?

I want to try doing a protein transfection experiment to target my protein of interest. there are are reagents like ProteoJuice which can be used. Does anyone have any experience or insight on the...

16 February 2017 627 3 View

Why we use different types of finger electrodes while depositing interdigitated electrodes in photodetector and why they use different dimensions?

e.g Finger electrodes made up of Ti/Al or Au/Cr(20nm/50nm) in MSM Photodetector

14 April 2016 1,526 6 View

Can we use a mask to fill the holes in an imge?

16 February 2016 2,591 7 View

Can relaxed circular plasmid be used for transformation? If yes, is it suitable to use such a plasmid for expression?

11 February 2015 5,831 2 View

How to decide the threshold levels in an LQAS methodology for a multiple indicator health project?

As in health system research, we will be measuring multiple indicators and we have to fix different threshold limits indicator wise. For some indicators the values will be less than 10 where the...

01 January 2014 6,728 2 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View

How to control the variants vof covid 19 in second and third waves?

A the world find means to control covid 19 by vaccination ,new variants came to spread and make the world in fear and danger, to understand the conduct of these variants, es constantly change...

21 February 2021 3,080 6 View

Surprisingly low antibody titers against SASRS-COV-2 RBD in hamsters compared to mice?

21 February 2021 6,958 2 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

How to calculate the TCID50 when the highest CPE is 50% or less?

I am doing TCID50 to measure progeny virus and some treatments protected the cells in a way that the highest CPE observed is 50% or less. How should I do the titre calculation in that case? Thank you!

16 February 2021 8,768 1 View

How to estimate/know plant virus concentration to make a standard curve in ELISA after serial dilution?

Hello, I am trying to know tomato virus titer/concentration in samples and I will perform serial dilution of the positive control. I wanted to ask How to estimate/know plant virus concentration to...

13 February 2021 6,534 4 View

What are the possible effects of COVID -19 Vaccine/s on the persons already recovered from the disease?

Persons recovered from COVID -19 are having antibodies against the virus inside their bodies. They are having T cells already sensitized to the antigens of the virus. If they are vaccinated (may...

12 February 2021 4,106 22 View

What is the reason, we got both test and control band on LFA in negative sample?

I am working on Lateral flow assay for virus detection by carbon conjugated polyclonal antibody. We got a high intense band in both test and control line on LFA in negative sample used as buffer....

11 February 2021 6,558 2 View