Immunization

Deliberate stimulation of the host's immune response. ACTIVE IMMUNIZATION involves administration of ANTIGENS or IMMUNOLOGIC ADJUVANTS. PASSIVE... | Review and cite IMMUNIZATION protocol, troubleshooting and other methodology information | Contact experts in IMMUNIZATION to get answers

4,387 views 117 posts

Questions related to Immunization

Can Immunogenic BNT162b vaccines protect HUMANS from SARS-CoV-2?

"A safe and effective vaccine against COVID-19 is urgently needed in quantities sufficient to immunise large populations. We report the preclinical development of two BNT162b vaccine candidates,...

12 February 2021 6,948 9 View

How to prepare a vial of Sigma Adjuvant System (Ribi) when needing multiple immunogens/at different immunisation points?

https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Datasheet/5/s6322dat.pdf Has anyone prepared a vial of sigma adjuvant systems (Ribi's) when they either aren't using the whole...

03 September 2020 7,717 2 View

Regarding Convalescent Plasma Therapy for COVID-19?

I had a few questions regarding Convalescent Plasma Therapy Why can't we use whole blood rather than convalescent plasma only? or Plasma + WBC (Adoptive cell transfer). This way we will transfer...

11 June 2020 2,609 9 View

% population of CD4 CD25 and FOXP3 for Control Vs Test groups?

After some reading and checking for the Treg cells during helminthic infection, I found my self somewhat confused for the % populations measured for CD4 CD25 and FOXP3 for Control against Text...

19 March 2019 7,182 3 View

Could e-card for immunisation be the innovation that could improve completion of immunisation among under 5 in the developing country?

There are challenges facing parents in keeping paper immunisation card in maintaining proper vaccination records for children under 5 years. The parents are faced with multitude of problems and...

28 January 2019 7,217 2 View

How to design and select the most relevant immunogenic peptide sequences.

What peptide sequences for immunisation will suitable? How to find out?

26 July 2017 7,724 4 View

Does treatment of r-protein with DTT impacts the mAB production?

I want to know if any of you has an idea about how much does the using of a DTT treated protein (as immunogen in mice) affects the mAb production process?

02 March 2017 9,459 4 View

Can I use precipitated or aggregated r-protein for immunization?

I will purify my r-protein (produced in E.coli) and cleave the MBP and His-tag off it and I have heard about the inclination of the r-protein to aggregate/precipitate when the tag cleavage takes...

01 March 2017 5,384 1 View

What would be a good read on how to perform a cost effectiveness study for a participartory action research?

I want to carry out a cost effectiveness study on a participatory action research aimed at improve immunisation coverage.

26 December 2016 1,306 5 View

Immunisation that induces immune response but does not gives protection?

Hi all, Goin thru the online papers that describe immunisation and challenge experiments normally draws to a conclusion that the immunisation gives protection or even partial protection to the...

20 December 2016 8,446 4 View

Mouse blood serum contamination and degradation at room T?

Hello everyone. I am doing immunization. after doing ELISA, i left serum collected from mice at room temperature for more than 10 hrs. Is there any chance that it is contaminated or my Antibody...

21 October 2016 8,448 3 View

Does anybody know a good adjuvant for peptide (20-mer) immunization to induce antibodies?

I would like to know a good adjuvant to combine with some peptides of 20aa long to immunize rabbits and after recovery of antibodies.

08 August 2016 1,022 6 View

Why Zika has not a huge impact in Africa compared to America?

We all know that Zika has circulated in Africa in the past and possibly conferred immunity in the populations of these settings. Except to that, I will look for having additional insights from all...

07 August 2016 8,451 4 View

Is it possible to extract RNA from commercial rabies vaccine?

I have tried extraction of RNA from various commercial rabies vaccine which currently used for pre exposure and post exposure for dogs. After that I have done RT- PCR, while running gel i got band...

02 August 2016 1,910 5 View

Monoclonal antibodies not detected in medium??

Dear All, I am working on development of hybridoma technology. I was used balb/C mice for immunization and SpO cell lines as myeloma cells. After 3rd booster spleen was removed aseptically....

04 July 2016 4,962 4 View

Has anyone immunized mice with Influenza HA1 antigen?

I would appreciate if anyone can share information about HA1 immunizations in mice. Particularly Cal09 HA1 in C57BL/6 mice or BALB/c mice. Any information about the murine MHC class2 restriction...

08 June 2016 3,895 3 View

Is there another way to look at immunization with protein band from SDS-PAGE?

In the late 1970s - beginning of the 1980s, we’d developed a pioneer approach to prepare antibodies against monooxygenase molecular isoforms without laborious purification of CYPs. To achieve the...

26 May 2016 7,582 1 View

New subunit E2 vaccine with celular respond after 7 days of immunization, one dose could be important?

I will appreciate very much your opinion

06 May 2016 3,603 1 View

Does OVA immunization elicit CD8 T cell response and generation of CTLs?

I collect splenic cells from C57/BL6 mice at day 7 post immunization with OVA protein and CFA, then incubate the sorted CD8 T cells from splenocytes with OVA257-264 pulsed EL4 target cells for...

05 April 2016 8,808 1 View

How Rabies vaccine potency test is calculated?

04 April 2016 5,917 3 View

What is the correct way to evaluate T-cell responses after immunization?

What is the correct way to evaluate T-cell responses after immunization? I am trying to determine the frequencies of antigen specific IFN-gamma-producing cells from the spleens of immunized mice...

13 October 2015 5,015 9 View

Can anybody recommend me a questionnaire regarding assessment of immunization status in children age 12-23 months?

I am doing my thesis on assessment of immunization status in children in District swat. I will be using multistage stratified sampling technique.This will be a house hold survey.

21 May 2015 4,733 5 View

May I know about conceptual framework of a pre test and post test research design for experimental study?

A study of the factors influencing the knowledge and attitude of mothers of under five children of a selected area of Kunderki, Moradabad U.P. regarding immunization and efficacy of a need based...

24 March 2015 7,687 4 View

Does anyone have experience looking at t follicular helper cells and b cells using flow cytometry?

I stained mouse lymph nodes with pd-1,cxcr5,cd4 and cd44 for t follicular helper cells and cd19,cd38,cd73 and af488 conjugated to an antigen that I originally immunized the mice with. I collected...

23 March 2015 6,582 1 View

Badges
Science topic