culture it

@Walma

The bright spots you’re seeing are likely contaminants, might be bacteria, fungal spores, or cellular debris.

In this case my suggestion is that:

1. do Gram staining or bacterial fluorescent stain to check if it’s bacterial or else?

2. Change to fresh sterile culture media to rule out contaminated supplies.

3. Assess cell viability just to ensure the issue isn’t due to the cell stress or death?