What is the the standard protocol/method for hemolysis assay?

27 October 2024 1 700 Report

Hey all

I want to prove that my nanoparticles are not toxic and do not lyse RBC. To establish this I have will have to do hemolysis assay.

I had looked several protocols on how to do hemolysis and several papers have mentioned to prepare a standard curve using hemoglobin standard and estimate the percentage of lysis. 'While some others have subtracted the absorbance from a the positive control such as 1% Trition X100 and calculated the relative percentage.

What is the standard protocol to be followed to do this assay?

What is ASTM standard E2524-08 (Standard Test Method for Analysis of Hemolytic Properties of Nanoparticles)

Jukka Salminen

ASTM standards are commercial products that can be bought from ANSI webstore https://webstore.ansi.org/standards/astm/astme2524082013

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