Introduction Cell counting and viability assessment are critical techniques in cellular biology and immunology. This protocol outlines the steps for counting mononuclear cells using a Neubauer chamber, as well as assessing cell viability using a dye exclusion method. The protocol will also provide guidance on calculating the volume needed to attain an exact number of cells per well in a culture plate.

Materials Required

Cell Counting Protocol

Calculating Volume Needed for Specific Cell Number per Well To calculate the volume required to achieve a specific number of cells per well (e.g., 1 x 10^6 cells per well), follow these steps:

Conclusion This protocol provides a systematic approach for counting mononuclear cells using a Neubauer chamber and assessing cell viability. By accurately calculating the volume needed to achieve a specific cell number per well, researchers can ensure consistent experimental conditions and reliable results in cellular studies. This protocol should help you in your research!