The organism used in the biofilm inhibition assay is Staphylococcus aureus. While performing the experiment in microtitre plate, I used 200 microlitres of (1:100 dilution of culture broth with absorbance 0.5). The incubation time was 24 hours. I could not observe biofilm formation at all even in the growth control when stained with crystal violet. Help me with suggestions as to how to improve the conditions for biofilm formation? Does increasing incubation time or using specialized broth help in biofilm formation by this bacteria?