I am looking for a protocol for bactearial decontamination in cell culture. Looks like bacillus for me. Is there any reference for that? There is no mycoplasma.
Hello Paula Mafra
Bacteria often produce toxins that can disrupt cellular function and destroy cultures. My suggestion is that you discard the contaminated culture immediately. If you notice the contamination early, it is possible to eradicate bacterial contamination with antibiotics. However, bacterial contamination is not easily treatable once the bacteria begin log phase of growth.
The commonly used antibiotics in cell culture are penicillin/streptomycin (must be used together), gentamycin, kanamycin and ampicillin. You should avoid using cocktails of many antibiotics because too many used together can result in a cumulative toxic effect on the cells. Antibiotics can change cell characteristics (metabolism) and create antibiotic-resistance.
Attached below are links that may help you treat bacterial contamination in cell culture.
https://unclineberger.org/tissueculture/contaminant/bacteriacontam/#:~:text=If%20a%20culture%20is%20contaminated,basic%20and%20will%20appear%20pink.
https://goldbio.com/documents/3983/Antibiotic%20Treatments%20of%20Microbe%20Contaminated%20Cell%20Cultures.pdf
Article Assessing and Controlling Microbial Contamination in Cell Cultures
My advice to you is do not waste your time in bacterial decontamination but rather concentrate on getting your lab and techniques in order by maintaining proper aseptic conditions so that you never face the problem of contamination.
Best.
Malcolm Nobre Sönke Weinert
Hi guys! Yes, I know it. Discarding is always the best solution.
However, it is a RARE strain, so in this case it is important to try. Of course, if it works, the ideal is to monitor the lineage to check for possible interferences.
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